Biotransformation of methyl-β-D-glucopyranoside to higher chain alkyl glucosides by cell bound β-glucosidase of Pichia etchellsii.
Mohd Younis Rather, Saroj Mishra, Vijeshwar Verma, Subhash Chand
Index: Bioresour. Technol. 107 , 287-294, (2012)
Full Text: HTML
Abstract
In the present study, we have investigated the use of Pichia etchellsii whole cells for synthesis of long-chain alkyl glucosides. Methyl-β-d-glucopyranoside (MG) was used in reaction with fatty alcohols, n-hexanol, n-octanol, n-decanol and n-dodecanol to synthesize the respective alkyl glucosides. The initial reaction conditions were first optimized at 2.5 ml scale for synthesis of octyl glucoside (OG) and were 8% water content, 100mM MG and 6h of reaction time and this resulted in ≈ 53% yield. A maximum transglucosylation/hydrolysis ratio of 2.79 was obtained at 100mM MG favoring high product yield. Based on the optimized conditions, a reactor was operated at 50 ml level which resulted in ≈ 60% conversion of MG to OG. A simple high performance liquid chromatography method was developed for quantitation of higher chain glucosides using a refractive index detector. A maximum of 27% and 13% yield was obtained for decyl-, and dodecyl-β-d-glucopyranoside, respectively.Copyright © 2011 Elsevier Ltd. All rights reserved.
Related Compounds
Related Articles:
Methylation in methanol-water mixtures: the effect of solvent composition and high pressure.
2008-05-01
[Biophys. Chem. 134 , 207-13, (2008)]
Sugar end-capped poly-D,L-lactides as excipients in oral sustained release tablets.
2009-01-01
[AAPS PharmSciTech 10(2) , 566-73, (2009)]
2012-04-01
[Biochim. Biophys. Acta 1823(4) , 971-82, (2012)]
Transport of glucose by Bifidobacterium animalis subsp. lactis occurs via facilitated diffusion.
2008-11-01
[Appl. Environ. Microbiol. 74(22) , 6941-8, (2008)]
2013-11-01
[J. Bacteriol. 195(21) , 4816-25, (2013)]