A sensitive capillary GC-MS method for analysis of topiramate from plasma obtained from single-dose studies.

Jeannine M Conway, Angela K Birnbaum, Susan E Marino, James C Cloyd, Rory P Remmel

Index: Biomed. Chromatogr. 26(9) , 1071-6, (2012)

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Abstract

Topiramate (Topamax®) is an antiepileptic medication used as adjunctive and monotherapy in patients with epilepsy and for migraine prophylaxis. A GC-MS assay was developed that was capable of detecting topiramate plasma concentrations following a single rectal or oral dose administration. Topiramate plasma samples were prepared by solid-phase extraction and were quantified by GC-MS analysis. The topiramate standard curves were split from 0.1 to 4 µg/mL and from 4 to 40 µg/mL in order to give a more accurate determination of the topiramate concentration. The accuracy of the standards ranged from 94.6 to 107.3% and the precision (%CV) ranged from 1.0 to 5.3% for both curves at all concentrations. The %CV for quality controls was <7.6%. The assay is both accurate and precise and will be used to complete future pharmacokinetic studies.Copyright © 2012 John Wiley & Sons, Ltd.