Analytical Biochemistry 1982-02-01

A specific stain for alpha-glucosidases in isoelectric focusing gels.

L L Spielman, D B Mowshowitz

Index: Anal. Biochem. 120 , 66, (1982)

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Abstract

α-Glucosidases separated on isoelectric focusing gels can be localized by a two-step staining procedure. First, bromonaphthyl-α- d-glucoside is cleaved by the α-glucosidases in the gel; then, the released bromonaphthol is precipitated with Fast Blue B salt. The procedure is specific for α-glucosidases, gives sharp enzyme bands, and does not inactivate the α-glucosidases. Using this procedure, it has been demonstrated that the maltase and α-methylglucosidase induced by maltose are separate enzymes.