A fluorometric assay for biotinidase.
H Ebrahim, K Dakshinamurti
Index: Anal. Biochem. 154 , 282, (1986)
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Abstract
An assay for biotinidase using biocytin, the natural substrate, is described. The fluorometric procedure uses 1,2-diacetylbenzene which reacts selectively with lysine allowing its direct determination in mixture with biocytin. We have examined the applicability of the assay using human serum biotinidase.
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