Immobilization of β-glucosidase on bifunctional periodic mesoporous organosilicas.
Liye Guan, Bin Di, Mengxiang Su, Jie Qian
Index: Biotechnol. Lett. 35(8) , 1323-30, (2013)
Full Text: HTML
Abstract
The aminopropyl-functionalized ethane-bridged bifunctional periodic mesoporous organosilicas (APEPMOs) were synthesized by the co-condensation of 1,2-bis (triethoxysilyl) ethane and 3-aminopropyltriethoxysilane in the presence of cationic surfactants octadecyltrimethylammonium chloride in basic medium. The pores of the APEPMOs were expanded with N,N-dimethyldecylamine and the pore-expanded materials were utilized as supports for β-glucosidase immobilization. A high enzyme loading of 120 mg per g support was achieved in 18 h, and 95.5 % of enzymatic activity was retained. β-Glucosidases were strongly immobilized on APEPMOs with only 5 % desorption in the washing step with buffer solution. The immobilized enzyme had 75 % activity after 20 batch reactions and had improved thermal stability, relative to the free enzyme. These results demonstrate that APEPMOs would be promising supports for β-glucosidases immobilization.
Related Compounds
Related Articles:
2012-10-01
[J. Sep. Sci. 35(20) , 2685-92, (2012)]
2010-05-15
[Talanta 81(3) , 824-30, (2010)]
Highly stable, anion conductive, comb-shaped copolymers for alkaline fuel cells.
2013-07-10
[J. Am. Chem. Soc. 135(27) , 10124-33, (2013)]
Hydroperoxide oxidations catalyzed by metals. II. Oxidation of tertiary amines to amine oxides. Sheng MN and Zajacek JG.
[J. Org. Chem. 33(2) , 588-590, (1968)]
Applications of pore-expanded MCM-41 silica: 4. Synthesis of a highly active base catalyst. Das DD, et al.
[Catal. Commun. 8(5) , 829-833, (2007)]