Analytical chemistry 2015-02-03

Highly sensitive method for specific, brief, and economical detection of glycoproteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis by the synthesis of a new hydrazide derivative.

Weitao Cong, Ayi Zhou, Zhiguo Liu, Jiayi Shen, Xuan Zhou, Weijian Ye, Zhongxin Zhu, Xinliang Zhu, Jianjun Lin, Litai Jin

Index: Anal. Chem. 87(3) , 1462-5, (2015)

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Abstract

A new hydrazide derivative was synthesized and used for the first time as a specific, brief, and economical probe to selectively visualize glycoproteins in 1-D and 2-D sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with high sensitivity. The detection limit of the newly developed staining method is 2- and 4-fold higher than that of the widely used Pro-Q Emerald 300 and 488 stains, respectively.