Separation and quantitation of Z-isomer in lanoconazole by normal phase HPLC.
A Phani Kumar, V R L Ganesh, D V Subba Rao, C Anil, V S Hariharakrishnan
Index: J. Pharm. Biomed. Anal. 50(3) , 535-7, (2009)
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Abstract
An isocratic normal phase high-performance liquid chromatographic (NP-HPLC) method has been developed and validated for the quantitation of Z-isomer in lanoconazole. Separation was achieved with a Thermo Hypersil Silica column. The ratio of 2-propanol, n-hexane and triethylamine in the mobile phase were optimized to obtain the best separation. UV detection was performed at 296 nm. The described method is linear over a range of LOQ--15.0 microg/ml of Z-isomer. The mean recovery of Z-isomer was found to be in the range of 97-99%. The method is simple, rapid, selective, accurate and precise, useful in the quality control of bulk manufacturing.
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