Simultaneous analysis of FDG, ClDG and Kryptofix 2.2.2 in [18F]FDG preparation by high-performance liquid chromatography with UV detection.
Ryuji Nakao, Takehito Ito, Masatoshi Yamaguchi, Kazutoshi Suzuki
Index: Nucl. Med. Biol. 35(2) , 239-44, (2008)
Full Text: HTML
Abstract
A practical, sensitive and rapid analytical method was established and validated for chemical impurity tests of 2-deoxy-2-fluoro-d-glucose (FDG), 2-deoxy-2-chloro-d-glucose (ClDG) and Kryptofix 2.2.2 (K-222) in [18F]FDG. This method was based on precolumn derivatization with ultraviolet (UV) detection. FDG and ClDG were rapidly derivatized with 1-phenyl-3-methyl-5-pyrazolone in the presence of borate buffer at 40 degrees C, and the labeled derivatives and K-222 were separated by reversed-phase high-performance liquid chromatography and monitored by UV absorbance at 210 nm. After optimization of the conditions, FDG, ClDG and K-222 could be determined within 15 min and showed good performance in terms of sensitivity, linearity and reproducibility. This method could be successfully applied to the quality control test of [18F]FDG produced by a commercially available apparatus.
Related Compounds
Related Articles:
1998-06-02
[Bioorg. Med. Chem. Lett. 8(11) , 1317-20, (1998)]
1986-01-01
[J. Membr. Biol. 89(3) , 251-67, (1986)]
Cation radius effects on the helix-coil transition of DNA. Cryptates and other large cations.
1990-04-01
[Biophys. J. 57(4) , 829-34, (1990)]
2007-12-01
[Appl. Radiat. Isot. 65(12) , 1359-62, (2007)]
2005-08-26
[J. Chromatogr. A. 1085(1) , 33-6, (2005)]