Journal of the American Society for Mass Spectrometry 1993-04-01

Matrix design for matrix-assisted laser desorption ionization: Sensitive determination of PAH-DNA adducts.

Z Dranitzki, C Shenberg, R Gale

Index: J. Am. Soc. Mass Spectrom. 5 , 1021-1025, (1994)

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Abstract

Two matrices, 4-phenyl-α-eyanocinnamic acid (PCC) and 4-benzyloxy-α-eyanocinnamic acid (BCC), were identified for the determination of polycyclic aromatic hydrocarbon (PAH) adducts of DNA bases by matrix-assisted laser desorption ionization. These matrices were designed based on the concept that the matrix and the analyte should have structural similarities. PCC is a good matrix for the desorption of not only PAH-modified DNA bases, but also PAHs themselves and their metabolites. Detections can be made at the femtomolar level.