Determination of imipramine in presence of iminodibenzyl and in pharmaceutical dosage form.

B A el-Zeany, A A Moustafa, N F Farid

Index: J. Pharm. Biomed. Anal. 33(4) , 775-82, (2003)

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Abstract

Two spectrophotometric methods for the determination of imipramine in presence of iminodibenzyl as an impurity are described. The first method is a ratio-spectra first derivative spectrophotometry, the signals were measured at 240.2 nm for imipramine. Calibration graph was found linear in the range 5-30 microg ml(-1). The second method is based on the reaction of imipramine base, being an electron donor, with p-chloranilic acid, being pi acceptor, to form a purple colored charge transfer complex. The absorbance was measured at 520.5 nm without interference with iminodibenzyl. Both methods are rapid, simple and do not require any preliminary separation or treatment of the samples. Furthermore, the two methods were applied to pharmaceutical dosage form.