Improved rapid assay of plasma uric acid by short-end injection capillary zone electrophoresis.

Salvatore Sotgia, Ciriaco Carru, Bastianina Scanu, Elisabetta Pisanu, Manuela Sanna, Gerard Aime Pinna, Leonardo Gaspa, Luca Deiana, Angelo Zinellu

Index: Anal. Bioanal. Chem 395 , 2577-2582, (2009)

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Abstract

A rapid and simple short-end injection capillary zone electrophoresis method was developed for the quantification of plasma uric acid. The separation was performed in an uncoated fused-silica capillary (50 microm ID, 60 cm total length, 10.2 cm effective length) by using as a background electrolyte a 75 mmol/L glycylglycine solution titrated with NaOH 5 mol/L to pH 9.0, a voltage of 28 kV, a cartridge temperature of 15 degrees C, and direct UV detection at 292 nm. Under optimized conditions, uric acid was determinate in little more than 1 min (1.076 minutes). In order to verify the accuracy of the analysis, urate levels were measured in 543 apparently healthy volunteers by the new assay and our previous method, and the obtained data were compared by Passing-Bablock regression, Bland-Altman test, and a new regression-based approach, which showed a good agreement between two methods.