Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses.
Johanna Bückers, Dominik Wildanger, Giuseppe Vicidomini, Lars Kastrup, Stefan W Hell
Index: Opt. Express 19(4) , 3130-43, (2011)
Full Text: HTML
Abstract
We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require a second STED beam, separating by lifetime is insensitive to drift and thus optimally suited for colocalization analyses. Furthermore, we propose a setup having a second STED beam for long duration multicolor recording.
Related Compounds
Related Articles:
Multistep energy transfer in single molecular photonic wires.
2004-06-02
[J. Am. Chem. Soc. 126 , 6514, (2004)]
2010-07-01
[Nucleic Acids Res. 38(13) , 4394-403, (2010)]
STED microscopy with a supercontinuum laser source.
2008-06-23
[Opt. Express 16(13) , 9614-21, (2008)]
A compact STED microscope providing 3D nanoscale resolution.
2009-10-01
[J. Microsc. 236(1) , 35-43, (2009)]
Y. Eberstein et al.
[J. Chem. Phys. B 108 , 93, (2004)]