P1,P4-diadenosine 5' tetraphosphate induces nitric oxide release from bovine aortic endothelial cells.

R H Hilderman, E F Christensen

Index: FEBS Lett. 427 , 320, (1998)

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Abstract

Since the infusion of P1,P4-diadenosine 5' tetraphosphate (Ap4A) into animal models induces vasodilation [1,2], the present study was performed to determine whether Ap4A induces the release of nitric oxide (NO) from endothelial cells. Ap4A induced NO release was 4.2-fold greater than the amount of NO released under basal condition. Ap4A induced NO release was inhibited by NG-nitro-L-arginine (L-NNA) and this inhibition was reversed by L-Arg. In addition, EGTA inhibits Ap4A induced NO release. These data are consistent with Ap4A inducing the release of NO from endothelial cells through the activation of endothelial nitric oxide synthase.