Metabolic labeling of prenyl and carboxyl-methyl groups.

A I Magee

Index: Curr. Protoc. Cell Biol. Chapter 7 , Unit 7.5, (2001)

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Abstract

This unit provides protocols for prenylation and carboxy-methylation of proteins in cultured cells. These modifications often accompany fatty acid acylation. Cultured cells can be labeled biosynthetically using radiolabeled mevalonate, a precursor, to label intermediates that are incorporated as prenoids--e.g., farnesyl and geranylgeranyl. Carboxy-methylation often accompanies prenylation. The methyl group can be labeled using [(3)H-methyl]methionine.