[Platinum tetrachloride coupled PEG-PEI as drug carrier and gene delivery vector].

Lu Zhao, Huan Chen, Dan Chen, Yi-Ping Hu, Hai Yu, Qing-Qing Wang, Gu-Ping Tang, Xiu-Rong Hu

Index: Zhejiang Da Xue Xue Bao. Yi Xue Ban 38(1) , 59-66, (2009)

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Abstract

To construct a drug carrier and gene vector PEG-PEI-Pt.Polyethyleneglycol (PEG) was coupled to polyethylenimine (PEI 600) and platinum tetrachloride; PEG-PEI-Pt complex was formed in ethanol. The complex was characterized by XRD, UV-VIS and FT-IR and the DNA condensation was tested by electrophoretic mobility shift assay. The cell viability was evaluated by MTT assay in Hela, B16, A293 and COS-7 cells and in vitro transfection efficiency was measured in A293 and B16 cells.The structure of PEG-PEI-Pt was characterized by XRD, UV-VIS and FT-IR. PEG-PEI-Pt complex was able to bind DNA at N/P weight ratio of 0.4:1; the complex showed cytotoxicity on Hela and B16 cells. The complex had higher transfection efficiency in A293 and B16 cells than PEI 600.A novel drug carrier and gene vector PEG-PEI-Pt was constructed successfully.