Psoralen preparation of antigenically intact noninfectious rotavirus particles.
W S Groene, R D Shaw, William S. Groene, Robert D. Shaw
Index: J. Virol. Methods 38(1) , 93-102, (1992)
Full Text: HTML
Abstract
The use of the synthetic psoralen 4'-aminomethyl-4,5',8-trimethylpsoralen hydrochloride (AMT) is described for the inactivation of infectious rotavirus, a member of the viral family Reoviradae with a double-stranded RNA genome. This method not only provides complete inactivation of the virus but leaves antigenically intact particles. The lack of viral replication following inactivation was determined with an immunohistochemical focus assay. The antigenic authenticity of the particles was determined by monoclonal antibody ELISA and a viral hemagglutination assay.
Related Compounds
Related Articles:
2007-11-01
[J. Gen. Virol. 88(Pt 11) , 3053-62, (2007)]
'RNA walk' a novel approach to study RNA-RNA interactions between a small RNA and its target.
2010-01-01
[Nucleic Acids Res. 38(1) , e5, (2010)]
Yeast U1 snRNP-pre-mRNA complex formation without U1snRNA-pre-mRNA base pairing.
2001-01-01
[RNA 7(1) , 133-42, (2001)]
1994-09-01
[Transfusion 34(9) , 802-10, (1994)]
1996-01-01
[Free Radic. Biol. Med. 20(5) , 751-6, (1996)]