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FBBBE

Names

[ CAS No. ]:
1522117-83-4

[ Name ]:
FBBBE

[Synonym ]:
3',6'-BIS{[4-(4,4,5,5-TETRAMETHYL-1,3,2-DIOXABOROLAN-2-YL)OXY]-3H-[SPIRO(ISOBENZOFURAN-1,9,-XANTHEN]-3-ONE}FBBBE

Biological Activity

[Description]:

FBBBE is used to detect the production of H2O2 by cells. FBBBE can be triggered by intracellular H2O2 and converted to fluorescein, resulting in an increase in intracellular fluorescence (Ex=4480 nm, Em=512 nm)[1].

[Related Catalog]:

Research Areas >> Cancer
Signaling Pathways >> Others >> Others
Research Areas >> Inflammation/Immunology

[Target]

H2O2[1]


[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1]. 1. The RAW 264.7 cells are seeded at 250,000 in glass bottom 35 mm dishes and post-adhering (5 h). 2. Some dishes are treated with 1 μg/mL of LPS for 24 h. Other dishes are treated the next day after plating with either 100 μM H2O2 for 1 h or PBS for 1 h. 3. Cells are washed twice with PBS and fixed in ice-cold 95% ethanol for 15 min. 3. Cells are twice rinsed with PBS and incubated with either 50 μM probe (FBBBE) for 1 h at room temperature. 4. Cells are washed twice with PBS and coverslips were mounted over cells using Aqua Mount. 5. Confocal images are obtained at 80× magnification on an Olympus FV1000 confocal laser scanning microscope. 6. Images were processed using ImageJ software.

[References]

[1]. Daniel, K.B., et al. Readily accessible fluorescent probes for sensitive biological imaging of hydrogen peroxide. Chembiochem 14(5), 593-598 (2013).

Chemical & Physical Properties

[ Density]:
1.3±0.1 g/cm3

[ Boiling Point ]:
595.2±60.0 °C at 760 mmHg

[ Molecular Formula ]:
C46H46B2O9

[ Molecular Weight ]:
584.229

[ Flash Point ]:
313.8±32.9 °C

[ Exact Mass ]:
584.238892

[ Appearance of Characters ]:
Powder

[ Vapour Pressure ]:
0.0±1.7 mmHg at 25°C

[ Index of Refraction ]:
1.600


Related Compounds