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WST-8

Names

[ CAS No. ]:
193149-74-5

[ Name ]:
WST-8

[Synonym ]:
Sodium 4-[3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl]-1,3-benzenedisulfonate
wst-8
2H-Tetrazolium, 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-, inner salt, sodium salt (1:1)

Biological Activity

[Description]:

WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.

[Related Catalog]:

Signaling Pathways >> Others >> Others
Biochemical Assay Reagents
Research Areas >> Others

[In Vitro]

The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×105 cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line[1].

[References]

[1]. Tiwari K, et al. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line. Pharm Biol. 2015 Jun;53(6):849-54.


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Chemical & Physical Properties

[ Molecular Formula ]:
C20H14N6NaO11S2

[ Molecular Weight ]:
601.48

[ PSA ]:
266.62000

[ LogP ]:
4.05220

[ Storage condition ]:
2~8°C

Safety Information

[ Hazard Codes ]:
C

Related Compounds