2-Nitrophenyl β-D-glucopyranoside

Names

[ Name ]:
2-Nitrophenyl β-D-glucopyranoside

[Synonym ]:
INDOXYL-GLUCOSIDE
Einecs 220-568-5
2-Nitrophenyl β-D-glucopyranoside
MFCD00065048
2-Nitrophenyl-β-D-glucopyranoside
β-D-Glucopyranoside, 2-nitrophenyl
2-Nitrophenyl b-D-glucopyranoside
o-Nitrophenyl β-D-glucopyranoside
O-NITROPHENYL B-D-GLUCOPYRANOSIDE
β-D-Glucopyranoside, 2-nitrophenyl (9CI)
2-NITROPHENYL-B-D-GLUCOSIDE

Biological Activity

[Description]:

2-Nitrophenyl β-D-glucopyranoside is a substrate for β-glucosidase. 2-Nitrophenyl β-D-glucopyranoside can be used to test β-glucosidase activity[1][2].

[Related Catalog]:

Research Areas >> Others

[In Vitro]

从 Cellulomonas biazotea 纯化的 β-glucosidase 对 2-Nitrophenyl β-D-glucopyranoside 的表观 Km 和 V 分别为 0.416 mM 和 0.22 U/mg 蛋白质[1]。

[References]

[1]. Siddiqui KS, et, al. Kinetic analysis of the active site of an intracellular beta-glucosidase fromCellulomonas biazotea. Folia Microbiol (Praha). 1997 Feb;42(1):53-8.

[2]. Nong H, et, al. Characterization of a novel β-thioglucosidase CpTGG1 in Carica papaya and its substrate-dependent and ascorbic acid-independent O-β-glucosidase activity. J Integr Plant Biol. 2010 Oct;52(10):879-90.

Chemical & Physical Properties

[ Density]:
1.6±0.1 g/cm3

[ Boiling Point ]:
572.4±50.0 °C at 760 mmHg

[ Molecular Formula ]:
C₁₂H₁₅NO₈

[ Molecular Weight ]:
301.249

[ Flash Point ]:
300.0±30.1 °C

[ Exact Mass ]:
301.079773

[ PSA ]:
145.20000

[ LogP ]:
-0.78

[ Vapour Pressure ]:
0.0±1.7 mmHg at 25°C

[ Index of Refraction ]:
1.648

[ Storage condition ]:
2-8°C

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi: Irritant;

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
S22-S24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Precursor & DownStream

Precursor

DownStream

Articles

Improving low-temperature catalysis in the hyperthermostable Pyrococcus furiosus beta-glucosidase CelB by directed evolution.

Biochemistry 39(13) , 3656-65, (2000)

The beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus (CelB) is the most thermostable and thermoactive family 1 glycosylhydrolase described to date. To obtain more insight in th...

Restoration of the activity of active-site mutants of the hyperthermophilic beta-glycosidase from Sulfolobus solfataricus: dependence of the mechanism on the action of external nucleophiles.

Biochemistry 37(49) , 17262-70, (1998)

The beta-glycosidase from the hyperthermophilic Archaeon Sulfolobus solfataricus hydrolyzes beta-glycosides following a retaining mechanism based upon the action of two amino acids: Glu387, which acts...

beta-Glucosidase as a reporter for the gene expression studies in Thermus thermophilus and constitutive expression of DNA repair genes.

Mutagenesis 21(4) , 255-60, (2006)

Thermus thermophilus is an extremely thermophilic eubacterium that grows optimally at 70-75 degrees C. Because the frequency of DNA damage, such as deamination, depurination and single-strand breaks, ...