β-Casomorphin, bovine

β-casomorphin, bovine (β-casomorphin-7) is a opioid peptide with an IC50 of 14 μM in an Opioid receptors binding assay.

Signaling Pathways >> GPCR/G Protein >> Opioid Receptor

Signaling Pathways >> Neuronal Signaling >> Opioid Receptor

Research Areas >> Metabolic Disease

Research Areas >> Neurological Disease

Peptides

IC50: 14 μM (Opioid receptor)[1]

β-casomorphin(β-casomorphin-7) strongly stimulates mucin secretion in the rat jejunum through a nervous pathway and opioid receptor activation. In rat DHE cells, β-casomorphin(β-casomorphin-7) increases the expression of rat mucin (rMuc)2 and rMuc3 but not rMuc1, rMuc4, and rMuc5AC. In human HT29-MTX cells, β-casomorphin(β-casomorphin-7) also increases MUC5AC mRNA levels (219% after 24 h of stimulation) and the secretion of this mucin. β-casomorphin(β-casomorphin-7) may contribute significantly to mucin production via a direct effect on intestinal goblet cells and the activation of -opioid receptors[2].

Oral administration of β-casomorphin(β-casomorphin-7) for 30 days to rats with STZ-induced diabetes results in a significant increase in serum level of insulin, and a decrease in the level of glucagon. The administration of β-casomorphin(β-casomorphin-7) alters the changes of SOD, GPx, T-AOC, MDA and H2O2 in the kidney of diabetic rats. β-casomorphin(β-casomorphin-7) alleviates high glucose-induced decreasement in SOD and GPx activity, increasement in MDA contents in the NRK-52E cells[3].

After 24 h of serum starvation, rat DHE cells are incubated without (control) or with β-casomorphin(β-casomorphin-7) (1-100 μM) for 30 min to 24 h. The amount of mucinlike glycoprotein in culture media is measured by enzymelinked lectin assay (ELLA) using biotinylated wheat germ agglutinin[2].

Rats[3] Diabetes is induced by intraperitoneal injection of STZ dissolved in 0.1 mol/L sodium citrate buffer (pH=4.0) at a dose of 60 mg/kg body weight. Fasting, tail-vein, blood glucose is measured three days after injection by using one touch glucomter, and Male Sprague-Dawley (SD) rats (200 g±10 g) that have a glucose level over 16.7 mM are considered to be diabetic rats. To investigate the preventive effects of β-casomorphin(β-casomorphin-7) treatment, β-casomorphin(β-casomorphin-7) treatment are carried out 2 weeks after the injection of STZ. All rats are euthanized after 30 days of treatment. On day 30, the animals are fasted overnight. Blood glucose, body and kidney weights are measured. Serum, plasma and kidney are collected for future analyses. All samples are stored at −70°C[3].

[1]. Brantl V, et al. Opioid activities of beta-casomorphins. Life Sci. 1981 Apr 27;28(17):1903-9.

[2]. Zoghbi S, et al. β-casomorphin-7-7 regulates the secretion and expression of gastrointestinal mucins through a μ-opioid pathway. Am J Physiol Gastrointest Liver Physiol. 2006 Jun;290(6):G1105-13.

[3]. Zhang W, et al. The protective effects of beta-casomorphin-7 against glucose -induced renal oxidative stress in vivo and vitro. PLoS One. 2013 May 3;8(5):e63472.

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