FICIN

Names

[ Name ]:
FICIN

[Synonym ]:
2-[(3-Aminopropanoyl)amino]-3-(1H-imidazol-4-yl)propanoic acid
Histidine, β-alanyl-
MFCD00081597
b-Alanylhistidine
β-Alanylhistidine
carnosine
N-(3-Aminopropanoyl)histidine

Biological Activity

[Description]:

Ficain is an enzyme extract composed of several proteases that can be isolated from Ficus hispida L. and the latex of fig (Ficus carica). Ficain has different specificities in different proportions during fruit ripening. Ficain is widely used in protein hydrolysis, food, production of bioactive peptides and antibody fragments[1][2].

[Related Catalog]:

Research Areas >> Others

Research Areas >> Metabolic Disease

[In Vitro]

Ficain (0-8 µg/mL) 以酪蛋白为底物，在 280 nm 处测量三氯乙酸滤液的吸光度，检测 Ficin 活性[2]。 Ficain (0-200 µg/mL) 在 p-nitrophenyl carbobenzoxyglycinate 为底物时，以剂量依赖性方式增加 400 nm 处的吸光度[2]。

[References]

[1]. Morellon-Sterling R, et al. Ficin: A protease extract with relevance in biotechnology and biocatalysis. Int J Biol Macromol. 2020 Nov 1;162:394-404.

[2]. Liener I E, Friedenson B. [17] Ficin[M]//Methods in enzymology. Academic Press, 1970, 19: 261-273.

[3]. RatanaKhanokchai K. Purification and Characterization of Cysteine Proteinase from Some Vegetables and fruits[J]. 2009.

Chemical & Physical Properties

[ Density]:
1.4±0.1 g/cm3

[ Boiling Point ]:
656.2±55.0 °C at 760 mmHg

[ Molecular Formula ]:
C₉H₁₄N₄O₃

[ Molecular Weight ]:
226.232

[ Flash Point ]:
350.7±31.5 °C

[ Exact Mass ]:
226.106583

[ LogP ]:
-2.17

[ Appearance of Characters ]:
saline suspension | tan

[ Vapour Pressure ]:
0.0±2.1 mmHg at 25°C

[ Index of Refraction ]:
1.591

[ Storage condition ]:
2-8°C

[ Water Solubility ]:
Soluble in cold water.

Safety Information

[ Symbol ]:

GHS08

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H334

[ Precautionary Statements ]:
P261-P342 + P311

[ Personal Protective Equipment ]:
Eyeshields;full-face respirator (US);Gloves;multi-purpose combination respirator cartridge (US);type ABEK (EN14387) respirator filter

[ Hazard Codes ]:
Xn,Xi

[ Risk Phrases ]:
36/37/38-42

[ Safety Phrases ]:
26-36-36/37-24-22-45-23

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ RTECS ]:
LK3675000

Articles

Various forms of rabbit plasma alpha-1-antiproteinase.

Biochem. Mol. Biol. Int. 46(1) , 27-34, (1998)

Amino acid sequencing of the ficin-derived C-terminal fragments of alpha-1-antiproteinase (also called alpha-1-antitrypsin or alpha-1-proteinase inhibitor) prepared from rabbit plasma revealed the pre...

Characterization of acid-induced molten globule like state of ficin.

Int. J. Biol. Macromol. 45(3) , 248-54, (2009)

Effect of pH on the conformational behaviour of ficin (EC 3.4.22.3), a cysteine protease from the latex of Ficus carica was monitored by circular dichroism, fluorescence spectroscopy, ANS binding and ...

Variation in aspects of cysteine proteinase catalytic mechanism deduced by spectroscopic observation of dithioester intermediates, kinetic analysis and molecular dynamics simulations.

Biochem. J. 357(Pt 2) , 343-52, (2001)

The possibility of a slow post-acylation conformational change during catalysis by cysteine proteinases was investigated by using a new chromogenic substrate, N-acetyl-Phe-Gly methyl thionoester, four...