3alpha-hydroxysteroid dehydrogenase

Names

[ Name ]:
3alpha-hydroxysteroid dehydrogenase

[Synonym ]:
ALPHA-HYDROXYSTEROID DEHYDROGENASE
3ALPHA-HYDROXYSTEROID: NAD[P]+ OXIDOREDUCTASE
EC 1.1.1.50
EC: 1.1.1.50
3alpha-hydroxysteroid dehydrogenase F. pseu. test. ~9 U/mg
3alpha-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni
Dehydrogenase, 3alpha-hydroxy steroid

Biological Activity

[Description]:

3α-Hydroxysteroid dehydrogenase (3α-HSD) is an enzyme encoded by the AKR1C4 gene, which can catalyze the conversion of 3-ketosteroids into 3α-hydroxy compounds. 3α-Hydroxysteroid dehydrogenase plays an important role in the inactivation of androgen DHT, and can convert DHT into 3α-androstanediol with weak androgen activity, which can be used in the research of hirsutism[1].

[Related Catalog]:

Research Areas >> Endocrinology

[References]

[1]. Anne Z Steiner, et al. 3alpha-Hydroxysteroid dehydrogenase type III deficiency: a novel mechanism for hirsutism. J Clin Endocrinol Metab. 2008 Apr;93(4):1298-303.

Chemical & Physical Properties

[ Appearance of Characters ]:
ammonium sulfate suspension

[ Storage condition ]:
2-8°C

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Safety Phrases ]:
24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Articles

Electronic effects of para-substitution on acetophenones in the reaction of rat liver 3alpha-hydroxysteroid dehydrogenase.

Bioorg. Med. Chem. 16 , 1084-9, (2008)

Stereoselective reductive metabolism of various p-substituted acetophenone derivatives was studied using isolated rat liver 3alpha-hydroxysteroid dehydrogenase (3alpha-HSD). Kinetic experiments were p...

Enzyme biosensor for androsterone based on 3α-hydroxysteroid dehydrogenase immobilized onto a carbon nanotubes/ionic liquid/NAD+ composite electrode.

Talanta 99 , 697-702, (2012)

A 3α-hydrosteroid biosensor for androsterone determination has been prepared by immobilizing the enzyme 3α-hydroxysteroid dehydrogenase (3α-HSD) in a composite electrode platform constituted of a mixt...

Prostaglandin dehydrogenase activity of purified rat liver 3 alpha-hydroxysteroid dehydrogenase.

Biochem. Biophys. Res. Commun. 148(2) , 646-52, (1987)

Homogeneous 3 alpha-hydroxysteroid dehydrogenase (3 alpha-HSD) from rat liver cytosol displays 9, 11, and 15-hydroxyprostaglandin dehydrogenase activity. Using [14C]-PGF2 alpha as substrate the produc...