Balanophonin

Balanophonin is an anti-inflammatory and anti-cancer agent. Balanophonin inhibits microglial activation and neurodegeneration via inhibiting activated microglia-induced apoptosis[1].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Signaling Pathways >> MAPK/ERK Pathway >> p38 MAPK

Research Areas >> Inflammation/Immunology

Signaling Pathways >> Immunology/Inflammation >> COX

Research Areas >> Neurological Disease

COX-2

p38

Balanophonin (1-10 μM; 24 h) 在 BV2 细胞中降低 Lipopolysaccharides (LPS; HY-D1056) 介导的 TLR4 激活和 NO 生成[1]。 Balanophonin (1-10 μM; 6 h) 降低 LPS 诱导的小胶质细胞中 iNOS 和 COX2 蛋白的表达以及 TNF-α 和 IL-1β 的产生[1]。 Balanophonin (1-10 μM; 30 min) 有效抑制 MAPK 激活[1]。 Balanophonin (1-10 μM; 24 h) 通过调节 N2a 细胞中裂解的 caspase-3 和聚 ADP 核糖聚合酶 (PARP) 的裂解，抑制 LPS 激活的小胶质细胞引起的神经元细胞死亡[1]。 Western Blot Analysis[1] Cell Line: LPS-activated BV-2 cells Concentration: 1, 5, and 10 μM Incubation Time: 30 min for MAPKs, 6 h for iNOS and COX2 and 24 h for PGE2, TNF-α and IL-1β Result: Downregulated TLR4. Reduced iNOS and COX2 expression. Significantly reduced the secretion of TNF-α and IL-1β. Decreased the phosphorylation of MAPKs such as pERK, pJNK, and p-p38. Western Blot Analysis[1] Cell Line: Neuroblastoma N2a cells Concentration: 1, 5, and 10 μM Incubation Time: 24 h Result: Slightly increased Bcl-2. Inhibited caspase-3 activation and PARP cleavage.

[1]. Lim SY, et al. A New Neolignan Derivative, Balanophonin Isolated from Firmiana simplex Delays the Progress of Neuronal Cell Death by Inhibiting Microglial Activation. Biomol Ther (Seoul). 2017 Sep 1;25(5):519-527.