6-O-Isobutyrylbritannilactone

6-O-Isobutyrylbritannilactone is a natural melanogenesis inhibitor. 6-O-Isobutyrylbritannilactone, a sesquiterpene, can be isolated from the flowers of Inula britannica. 6-O-Isobutyrylbritannilactone inhibits IBMX (HY-12318)-induced melanin production in B16F10 cells. 6-O-Isobutyrylbritannilactone also regulates ERK, PI3K/AKT, and CREB, shows antimelanogenic activity in zebrafish embryos models[1].

Research Areas >> Cancer

Signaling Pathways >> PI3K/Akt/mTOR >> PI3K

Signaling Pathways >> PI3K/Akt/mTOR >> Akt

Signaling Pathways >> MAPK/ERK Pathway >> ERK

Signaling Pathways >> Epigenetics >> Epigenetic Reader Domain

Signaling Pathways >> Stem Cell/Wnt >> ERK

6-O-Isobutyrylbritannilactone (5-100 μM; 48 h) 对 IBMX (100 μM; 24 h)[1]。 6-O-Isobutyrylbritannilactone (5-30 μM; 12 h) 剂量依赖性地抑制 IBMX 刺激 B16F10 细胞的白化相关 mRNA 水平 (100 μM; 48 h)[1]。 6-O-Isobutyrylbritannilactone (20 μM; 1-9 h) 时间依赖性地抑制 ERK、AKT 和 CREB 的磷酸化[1]。 RT-PCR[1] Cell Line: B16F10 cells Concentration: 5 μM, 10 μM, 20 μM, 30 μM Incubation Time: 12 hours; with 100 μM IBMX for 48 hours Result: Inhibited whitening-related mRNA levels, and resulted in completely inhibited expressions of melanogenesis-related protein levels at 20 μM. Western Blot Analysis[1] Cell Line: 20 μM Concentration: 5 μM, 10 μM, 20 μM, 30 μM Incubation Time: 1 h, 3 h, 6 h, and 9 h Result: Inhibited the phosphorylation of ERK, AKT, and CREB. Demonstrated the melanogenesis suppression induced by IBMX via inaction of multiple signaling pathways. Cell Cytotoxicity Assay[1] Cell Line: B16F10 cells Concentration: 5 μM, 10 μM, 20 μM, 50 μM, and 100 μM Incubation Time: 24 hours Result: Inhibited B16F10 cells viability in a dose-dependent manner.

6-O-Isobutyrylbritannilactone (10-100 μM; 48 h) 与未处理的对照组相比，显著减少斑马鱼胚胎中的色素沉着[1]。 Animal Model: Zebrafish embryos[1] Dosage: 10 μM, 50 μM, and 100 μM Administration: Dipped in culture for 48 hours Result: Reduced pigmentation by approximately 8%, 13%, and 16%.

[1]. Jang DK, et al. Anti-Melanogenesis Activity of 6-O-Isobutyrylbritannilactone from Inula britannica on B16F10 Melanocytes and In Vivo Zebrafish Models. Molecules. 2020 Aug 26;25(17):3887.