Name | NAI-N3 |
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Description | NAI-N3 is a novel RNA acylation reagent that enables RNA purification. |
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Related Catalog | |
In Vitro | The bifunctional NAI-N3 SHAPE reagent is used to label all RNAs inside living cells (or in vitro refolded RNA) to generate covalent modifications on targeted RNAs at single-nucleotide resolution. RNA is isolated, biotinylated using click chemistry and randomly fragmented to a size of 50–100 nt. To generate cDNA sequencing libraries, the RNA fragments are subjected to 3′-end repair, adapter ligation, size selection, biotin enrichment, reverse transcription (RT) and PCR. The end result is selective amplification of fragments corresponding to modified bases in the NAI-N3–treated samples or background expression values from mock-treated samples. Sequencing reads generated from the NAI-N3– and mock (DMSO)-treated samples are mapped to a transcriptome annotation, followed by computational identification of RT stops corresponding to SHAPE modifications—and hence reactive nucleotides—across the entire transcriptome[1]. |
References |
Molecular Formula | C10H8N6O |
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Molecular Weight | 228.21 |
Storage condition | 2-8℃ |