139554-72-6
139554-72-6 structure
- Name: N-BIOTINYL-N'-MALEIMIDO-ETHYLENEDIAMINE
- Chemical Name: N-BIOTINYL-N'-MALEIMIDO-ETHYLENEDIAMINE
- CAS Number: 139554-72-6
- Molecular Formula: C16H23N5O4S
- Molecular Weight: 381.45
- Catalog: Research Areas Cancer
- Create Date: 2018-06-25 00:10:29
- Modify Date: 2024-01-19 15:14:40
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Biotin-C2-maleimide is an antibody conjugate reagent, can bind to many biomolecules without significantly changing the biological activity of the target molecule[1][2].
| Name | N-BIOTINYL-N'-MALEIMIDO-ETHYLENEDIAMINE |
|---|
| Description | Biotin-C2-maleimide is an antibody conjugate reagent, can bind to many biomolecules without significantly changing the biological activity of the target molecule[1][2]. |
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| Related Catalog | |
| In Vitro | Biotin-C2-maleimide () can act bioconjugation with 5 eq. SUMO-1 probes[1]. 1. Biotin C2 maleimide (ATT Bioquest, dissolved in DMSO) is performed in Tris buffer (50 mM Tris, 300 mM NaCl; pH 7.0) after 15 min reduction with Tris (2-carboxyethyl)phosphine (TCEP, 120 µM) at room temperature. 2. After 1 h incubation, the unreacted Biotin is separated by Ni-NTA affinity chromatography followed by dialysis against PBS buffer (pH 7.4). |
| References |
| Melting Point | 123-129°C |
|---|---|
| Molecular Formula | C16H23N5O4S |
| Molecular Weight | 381.45 |
| Storage condition | -20°C Freezer, Under Inert Atmosphere |