24696-26-2

24696-26-2 structure

Name: c16 ceramide
Chemical Name: N-(1,3-dihydroxyoctadec-4-en-2-yl)hexadecanamide
CAS Number: 24696-26-2
Molecular Formula: C₃₄H₆₇NO₃
Molecular Weight: 537.90100
Catalog: Chemical reagent Organic reagent Fatty alcohol
Create Date: 2018-07-24 18:56:55
Modify Date: 2024-01-03 18:36:12
C16-Ceramide is a natural small molecule activating p53 through the direct and selective binding[1].

Name	N-(1,3-dihydroxyoctadec-4-en-2-yl)hexadecanamide
Synonyms	C16 CERAMIDE N-(hexadecanoyl)-sphing-4-enine N-Palmitoyl-D-sphingosine N-PALMITOYL-D-SPHINGOSINE C16-ceramide N-Palmitoyl-D-erythro-sphingosine Palmitoyl ceramide N-Hexadecanoyl-D-sphingosine

Description	C16-Ceramide is a natural small molecule activating p53 through the direct and selective binding[1].
Related Catalog	Signaling Pathways >> Apoptosis >> Apoptosis Research Areas >> Cancer Signaling Pathways >> Apoptosis >> MDM-2/p53
Target	Human Endogenous Metabolite
In Vitro	C16-Ceramide interacts with p53 within its core domain. p53 forms complex with natural C16-Ceramide in the cell[1]. C16-Ceramide (2.5-50 μM; 0-48 h) strongly decreased HCT116 cell viability in a time- and concentration-dependent manner[2]. C16-Ceramide (12 μM; 48 h) induces apoptosis through Btf (Bcl-2-associated transcription factor) in HCT116 cells[2]. C16-ceramide (12 μM; 0-6 h) and Btf expression up-regulate p53 and BAX expression. C16-ceramide down-regulates Mdm2 expression via Btf[2]. Cell Viability Assay[2] Cell Line: HCT116 cells Concentration: 2.5, 5, 10, 12, 20, 50 µM Incubation Time: 0-48 h Result: Strongly decreased cell viability in a time- and concentration-dependent manner. Western Blot Analysis[2] Cell Line: HCT116 cells Concentration: 12 μM Incubation Time: 1, 3 and 6 h Result: Increased PARP cleavage, decreased pro-caspase 3. Decreased the levels of stratifin and stathmin, increased the expression of prohibitin and Btf. RNAi-mediated Btf depletion alsopartially inhibited BAX expression after the treatment. Significantly decreased luciferase activity and Mdm2 protein expression levels.