Mast Cell Degranulating (MCD) Peptide

Modify Date: 2024-01-08 07:21:35

Mast Cell Degranulating (MCD) Peptide structure	Common Name	Mast Cell Degranulating (MCD) Peptide
	CAS Number	17466-45-4	Molecular Weight	788.86800
	Density	1.51g/cm3	Boiling Point	1370.5ºC at 760mmHg
	Molecular Formula	C₃₅H₄₈N₈O₁₁S	Melting Point	N/A
	MSDS	Chinese USA	Flash Point	782.6ºC
	Symbol	GHS06	Signal Word	Danger

Use of Mast Cell Degranulating (MCD) Peptide

Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome(λex=495 nm, λem=520 nm). Sequence: Ala-{d-Thr}-Cys-{Hyp}-Ala-Trp-Leu (Disulfide bridge: Cys3-Cys6);A-{d-Thr}-C-{Hyp}-AWL (Disulfide bridge: Cys3-Cys6).

Name	phalloidin
Synonym	More Synonyms

Description	Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome(λex=495 nm, λem=520 nm). Sequence: Ala-{d-Thr}-Cys-{Hyp}-Ala-Trp-Leu (Disulfide bridge: Cys3-Cys6);A-{d-Thr}-C-{Hyp}-AWL (Disulfide bridge: Cys3-Cys6).
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others Peptides
In Vitro	Phalloidin staining will not work with methanol-fixed cells, probably because of the disruption of actin filament integrity, but it will work with cells fixed in 0.2% glutaraldehyde in PBS. For fluorescein isothiocyanate (FITC)-Phalloidin (or rhodamine isothiocyanate [RITC]-Phalloidin), the excitation wavelength maximum will be near 495 nm (or 550 nm) and the emission wavelength maximum will be ~520 nm based on H2O at pH 8.0 as the solvent (575 nm with methanol as the solvent). The emission spectrum of fluorescein can depend on the environmental pH and/or ionic strength[1].
Cell Assay	Prepare a 1:200 dilution of the stock solution (300 units/mL) of fluorescein Phalloidin or rhodamine Phalloidin using PBS. Aspirate the cell medium from the cells grown on glass coverslips and rinse the cells three times with PBS. Fix the cells for 10 min in the 3.7% formaldehyde solution. Rinse the fixed cells three times for 5 min each in PBS. Permeabilize the cells for 5 min in the 0.2% Triton X-100 solution. Rinse the fixed cells three times with PBS. Label the cells with the fluorescein or rhodamine Phalloidin for 5 to 10 min at room temperature. Rinse the cells three times in PBS for 5 min each time. Mount the cell and view the cells using either the fluorescein or rhodamine filter set, depending on the probe[1].
References	[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947.

Density	1.51g/cm3
Boiling Point	1370.5ºC at 760mmHg
Molecular Formula	C₃₅H₄₈N₈O₁₁S
Molecular Weight	788.86800
Flash Point	782.6ºC
Exact Mass	788.31600
PSA	316.92000
Vapour Pressure	0mmHg at 25°C
Index of Refraction	1.683

Mast Cell Degranulating (MCD) Peptide MSDS(Chinese)

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SE9800000

CHEMICAL NAME :: Phalloidin

CAS REGISTRY NUMBER :: 17466-45-4

LAST UPDATED :: 199612

DATA ITEMS CITED :: 8

MOLECULAR FORMULA :: C35-H48-N8-O11-S

MOLECULAR WEIGHT :: 788.97

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 1 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 2 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 6600 ug/kg

TOXIC EFFECTS :: Behavioral - muscle weakness Liver - fatty liver degeneration

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Unreported

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 2 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD - Lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Mammal - dog

DOSE/DURATION :: >10 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Mammal - species unspecified

DOSE/DURATION :: 1000 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

MUTATION DATA

TYPE OF TEST :: DNA inhibition

TEST SYSTEM :: Rodent - rat Liver

DOSE/DURATION :: 100 nmol/L

REFERENCE :: TOXIA6 Toxicon. (Pergamon Press Ltd., Headington Hill Hall, Oxford OX3 OBW, UK) V.1- 1962- Volume(issue)/page/year: 25,1265,1987

Symbol	GHS06
Signal Word	Danger
Hazard Statements	H300 + H310 + H330
Precautionary Statements	P260-P264-P280-P284-P301 + P310-P302 + P350
Hazard Codes	T+
Risk Phrases	26/27/28
Safety Phrases	S22;S28;S45;S36/S37
RIDADR	UN 3462 6.1/PG 2
RTECS	SE9800000
Hazard Class	6.1(a)

Precursor 0
DownStream 2
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EINECS 241-484-5

MFCD03427567

PHALLOIDIN

Phalloidine

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