PNPP

Modify Date: 2024-01-02 21:40:07

PNPP Structure
PNPP structure
Common Name PNPP
CAS Number 4264-83-9 Molecular Weight 219.089
Density 1.7±0.1 g/cm3 Boiling Point 457.8±47.0 °C at 760 mmHg
Molecular Formula C6H4NNa2O6P Melting Point >300 °C(lit.)
MSDS Chinese USA Flash Point 230.7±29.3 °C
Symbol GHS05 GHS08
GHS05, GHS08
Signal Word Danger

 Use of PNPP


PNPP is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

 Names

Name Disodium 4-nitrophenylphosphate
Synonym More Synonyms

 PNPP Biological Activity

Description PNPP is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.
Related Catalog
In Vitro Phosphatases catalyze the hydrolysis of PNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP). The resulting phenolate is yellow, with a maximal absorption at 405 nm[1].

 Chemical & Physical Properties

Density 1.7±0.1 g/cm3
Boiling Point 457.8±47.0 °C at 760 mmHg
Melting Point >300 °C(lit.)
Molecular Formula C6H4NNa2O6P
Molecular Weight 219.089
Flash Point 230.7±29.3 °C
Exact Mass 218.993271
PSA 128.05000
LogP 0.37
Vapour Pressure 0.0±1.2 mmHg at 25°C
Index of Refraction 1.617
Storage condition 2-8°C
Stability Stable, but may be light sensitive. Incompatible with strong acids, strong bases, strong oxidizing agents.
Water Solubility H2O: 1 tablet/3 mL 25 mM, clear, light yellow

 Safety Information

Symbol GHS05 GHS08
GHS05, GHS08
Signal Word Danger
Hazard Statements H290-H315-H318-H373
Precautionary Statements P260-P280-P305 + P351 + P338 + P310
Hazard Codes Xi: Irritant;
Risk Phrases R36/37/38
Safety Phrases S26-S36-S24/25-S23
RIDADR NONH for all modes of transport
WGK Germany 3
HS Code 2919900090

 Precursor & DownStream

Precursor  1

DownStream  1

 Customs

HS Code 2919900090
Summary 2919900090 other phosphoric esters and their salts, including lactophosphates; their halogenated, sulphonated, nitrated or nitrosated derivatives。supervision conditions:AB(certificate of inspection for goods inward,certificate of inspection for goods outward)。VAT:17.0%。tax rebate rate:9.0%。MFN tariff:6.5%。general tariff:30.0%

 Articles188

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Enhancement of enzymatic colorimetric response by silver island films on high throughput screening microplates.

J. Immunol. Methods 411 , 43-9, (2014)

In this study, we report the use of an enzyme-based hybrid platform, which is comprised of silver island films, enzymes (HRP and AP) and high-throughput screening (HTS) microplates, to enhance the col...

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Biotechnol. Bioeng. 112(2) , 308-21, (2015)

Tobacco BY-2 cells have emerged as a promising platform for the manufacture of biopharmaceutical proteins, offering efficient protein secretion, favourable growth characteristics and cultivation in co...

Sprayed cells and polyelectrolyte films for biomaterial functionalization: the influence of physical PLL-PGA film treatments on dental pulp cell behavior.

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Further development of biomaterials is expected as advanced therapeutic products must be compliant to good manufacturing practice regulations. A spraying method for building-up polyelectrolyte films f...

 Synonyms

4-Nitrophenyl dihydrogen phosphate
disodium,(4-nitrophenyl) phosphate
4-Nitrophenylphosphoric Acid Disodium Salt
4-Nitrophenyl phosphate disodium salt
Phosphoric acid mono(4-nitrophenyl) ester
4-Nitrophenyl phosphate disodium salt hexahydrate
p-Nitrophenyl Phosphate Liquid Substrate System
p-nitro-Phenol dihydrogen phosphate
4-Nitrophenyl phosphate,disodium salt,hexahydrate
para-Nitrophenylphosphate
EINECS 224-246-5
MFCD00007319
Phosphoric acid mono(p-nitrophenyl) ester
Phosphoric acid, mono(4-nitrophenyl) ester
Sodium 4-nitrophenyl phosphate
PNPP
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