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鬼笔环肽

鬼笔环肽用途

Phalloidin 是一种蘑菇衍生的毒素,可以与荧光染料结合来标记细胞骨架的 F-肌动蛋白 (λex=495 nm, λem=520 nm)。

鬼笔环肽名称

[ CAS 号 ]:
17466-45-4

[ 中文名 ]:
毒伞素

[ 英文名 ]:
Mast Cell Degranulating (MCD) Peptide

[英文别名 ]:

EINECS 241-484-5
MFCD03427567
PHALLOIDIN
Phalloidine
Phalloidin from Amanita phalloides

鬼笔环肽生物活性

[ 描述 ]:

Phalloidin 是一种蘑菇衍生的毒素,可以与荧光染料结合来标记细胞骨架的 F-肌动蛋白 (λex=495 nm, λem=520 nm)。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

多肽产品

[体外研究]

鬼笔环肽染色不适用于甲醇固定的细胞,可能是因为肌动蛋白丝完整性的破坏,但它适用于固定在PBS中的0.2％戊二醛中的细胞。对于异硫氰酸荧光素(FITC)-多倍体蛋白(或罗丹明异硫氰酸酯[RITC] -多倍体蛋白),激发波长最大值将接近495nm(或550nm),并且基于pH8.0的H2O,发射波长最大值将为~520nm。溶剂(575nm,甲醇作为溶剂)。荧光素的发射光谱可以取决于环境pH和/或离子强度[1]。

[细胞实验]

使用PBS制备1：200稀释的荧光素鬼笔环肽或罗丹明鬼笔环肽的储备溶液（300单位/ mL）。从玻璃盖玻片上生长的细胞中吸出细胞培养基，用PBS冲洗细胞三次。将细胞在3.7％甲醛溶液中固定10分钟。在PBS中冲洗固定的细胞三次，每次5分钟。在0.2％Triton X-100溶液中使细胞透化5分钟。用PBS冲洗固定的细胞三次。在室温下用荧光素或罗丹明鬼笔环肽标记细胞5至10分钟。每次在PBS中冲洗细胞三次，每次5分钟。根据探头[1]，使用荧光素或罗丹明滤光片组安装细胞并观察细胞。

[参考文献]

[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947.

[相关活性小分子]

鬼笔环肽物理化学性质

[ 密度 ]:
1.51g/cm3

[ 沸点 ]:
1370.5ºC at 760mmHg

[ 分子式 ]:
C₃₅H₄₈N₈O₁₁S

[ 分子量 ]:
788.86800

[ 闪点 ]:
782.6ºC

[ 精确质量 ]:
788.31600

[ PSA ]:
316.92000

[ 外观性状 ]:
白色粉末

[ 蒸汽压 ]:
0mmHg at 25°C

[ 折射率 ]:
1.683

[ 储存条件 ]:
库房低温,通风,干燥,与食品原料分开存放

鬼笔环肽MSDS

详细MSDS(安全技术说明书)

鬼笔环肽毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SE9800000

CHEMICAL NAME :: Phalloidin

CAS REGISTRY NUMBER :: 17466-45-4

LAST UPDATED :: 199612

DATA ITEMS CITED :: 8

MOLECULAR FORMULA :: C35-H48-N8-O11-S

MOLECULAR WEIGHT :: 788.97

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 1 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 2 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 6600 ug/kg

TOXIC EFFECTS :: Behavioral - muscle weakness Liver - fatty liver degeneration

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Unreported

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 2 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD - Lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Mammal - dog

DOSE/DURATION :: >10 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Mammal - species unspecified

DOSE/DURATION :: 1000 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

MUTATION DATA

TYPE OF TEST :: DNA inhibition

TEST SYSTEM :: Rodent - rat Liver

DOSE/DURATION :: 100 nmol/L

REFERENCE :: TOXIA6 Toxicon. (Pergamon Press Ltd., Headington Hill Hall, Oxford OX3 OBW, UK) V.1- 1962- Volume(issue)/page/year: 25,1265,1987

鬼笔环肽安全信息

[ 符号 ]:

GHS06

[ 信号词 ]:
Danger

[ 危害声明 ]:
H300 + H310 + H330

[ 警示性声明 ]:
P260-P264-P280-P284-P301 + P310-P302 + P350

[ 危害码 (欧洲) ]:
T+

[ 风险声明 (欧洲) ]:
26/27/28

[ 安全声明 (欧洲) ]:
S22;S28;S45;S36/S37

[ 危险品运输编码 ]:
UN 3462 6.1/PG 2

[ RTECS号 ]:
SE9800000

[ 危险类别 ]:
6.1(a)

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鬼笔环肽文献

The Qdot-labeled actin super-resolution motility assay measures low-duty cycle muscle myosin step size.

Biochemistry 52(9) , 1611-21, (2013)

Myosin powers contraction in heart and skeletal muscle and is a leading target for mutations implicated in inheritable muscle diseases. During contraction, myosin transduces ATP free energy into the w...

Ionomycin causes susceptibility to phospholipase A2 while temperature-induced increases in membrane fluidity fail: possible involvement of actin fragmentation.

Biochim. Biophys. Acta 1838(10) , 2607-14, (2014)

A diminution in the order of membrane lipids, which occurs during apoptosis, has been shown to correlate with increased membrane susceptibility to hydrolysis by secretory phospholipase A2. Studies wit...

Visualizing myosin-actin interaction with a genetically-encoded fluorescent strain sensor.

Proc. Natl. Acad. Sci. U. S. A. 105(44) , 16882-7, (2008)

Many proteins have been shown to undergo conformational changes in response to externally applied force in vitro, but whether the force-induced protein conformational changes occur in vivo remains unc...

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