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异硫氰酸荧光素

异硫氰酸荧光素用途

5(6)-FITC(FITC)是一种胺活性衍生物的荧光染料,具有广泛的应用,作为抗体和其他探针标记,用荧光显微镜,流式细胞仪、免疫荧光方法如ELISA和Western印迹实验。

异硫氰酸荧光素名称

[ CAS 号 ]:
27072-45-3

[ 中文名 ]:
异硫氰酸荧光素

[ 英文名 ]:
fluorescein 5-isothiocyanate

[中文别名 ]:

[英文别名 ]:

5-FITC
Isothiocyanatofluorescein
5-Isothionatofluorescein
FITC isomer I
Fluorescein Isothiosyanate
fluorescein isothiocyanate isomer 1
FITC ISOMER
EINECS 222-042-0
FITC-I
Fluorescein 5(6)-isothiocyanate

异硫氰酸荧光素生物活性

[ 描述 ]:

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[参考文献]

[1]. Goding JW. Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Methods. 1976;13(3-4):215-26.

[2]. Der-Balian GP, et al. Fluorescein labeling of Fab' while preserving single thiol. Anal Biochem. 1988 Aug 15;173(1):59-63.

[3]. Smith LM, et al. Fluorescence detection in automated DNA sequence analysis. Nature. 1986 Jun 12-18;321(6071):674-9.

[相关活性小分子]

异硫氰酸荧光素物理化学性质

[ 密度 ]:
1.5±0.1 g/cm3

[ 沸点 ]:
708.6±60.0 °C at 760 mmHg

[ 熔点 ]:
>360ºC(lit.)

[ 分子式 ]:
C₂₁H₁₁NO₅S

[ 分子量 ]:
389.381

[ 闪点 ]:
382.4±32.9 °C

[ 精确质量 ]:
389.035797

[ PSA ]:
120.44000

[ LogP ]:
4.00

[ 外观性状 ]:
powder

[ 蒸汽压 ]:
0.0±2.3 mmHg at 25°C

[ 折射率 ]:
1.754

[ 储存条件 ]:
2-8°C

异硫氰酸荧光素MSDS

详细MSDS(安全技术说明书)

异硫氰酸荧光素安全信息

[ 符号 ]:

GHS08

[ 信号词 ]:
Danger

[ 危害声明 ]:
H334

[ 警示性声明 ]:
P261-P342 + P311

[ 个人防护装备 ]:
dust mask type N95 (US);Eyeshields;Faceshields;Gloves

[ 危害码 (欧洲) ]:
Xn: Harmful;

[ 风险声明 (欧洲) ]:
R42/43

[ 安全声明 (欧洲) ]:
S22

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

异硫氰酸荧光素制备

由4-硝基邻苯二甲酸酐（见C8H3NO5，[5466-84-2]）与间苯二酚在195-200℃反应10h，环合为3'-硝基荧光黄和4'-硝基荧光黄的混合物，再用乙酐乙酰化，加饱和氢氧化钠-乙醇溶液水解，得4'-硝基荧光素。然后将4'-硝基荧光素溶解在氨水中，加雷氏镍及水合肼室温反应2h，还原为4'-氨基荧光素。最抬经酰化，消除得到异硫氰酸荧光素。

异硫氰酸荧光素文献

Imaging of a clinically relevant stroke model: glucose hypermetabolism revisited.

Stroke 46(3) , 835-42, (2015)

Ischemic stroke has been shown to cause hypermetabolism of glucose in the ischemic penumbra. Experimental and clinical data indicate that infarct-related systemic hyperglycemia is a potential therapeu...

Surface modifications of silica nanoparticles are crucial for their inert versus proinflammatory and immunomodulatory properties.

Int. J. Nanomedicine 9 , 2815-32, (2014)

Silica (SiO₂) nanoparticles (NPs) are widely used in diverse industrial and biomedical applications. Their applicability depends on surface modifications, which can limit potential health problems.To ...

Uptake of neutrophil-derived Ym1 protein distinguishes wound macrophages in the absence of interleukin-4 signaling in murine wound healing.

Am. J. Pathol. 184(12) , 3249-61, (2014)

The determination of regenerative wound-healing macrophages as alternatively activated macrophages is currently questioned by the absence of IL-4 in wound tissue. Yet, murine wound tissue expressed hi...

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