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CBIC2

CBIC2用途

JC-1是荧光亲脂性羰花青染料,用于测量线粒体膜电位。

CBIC2名称

[ CAS 号 ]:
3520-43-2

[ 中文名 ]:
5,5’,6,6’-四氯-1,1’,3,3’-四乙基苯并咪唑羰花青碘化物

[ 英文名 ]:
JC-1

[中文别名 ]:

[英文别名 ]:

JC-1

CBIC2生物活性

[ 描述 ]:

JC-1是荧光亲脂性羰花青染料,用于测量线粒体膜电位。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

暴露于鼠L1210淋巴母细胞的JC-1(2.5μM)可检测到这些细胞中细胞质JC-1单体和线粒体J-聚集体的存在。 JC-1荧光通常由流式细胞仪中常见的488 nm激光波长激发[1]。用JC-1(1μg/ mL,15分钟)荧光标记线粒体,显示出不规则分布,导致星形胶质细胞内线粒体含量高和低的区域[2]。已显示JC-1在酸性C-末端区域与α-突触核蛋白相互作用,Kd为2.6μM。 JC-1本身不会在没有铁的情况下加速α-突触核蛋白的蛋白质聚集,因此,它通过延长滞后期来减缓聚集过程[3]。 JC-1在敏感的K562细胞中积累,其中显示绿色细胞质和红色线粒体荧光。 JC-1在抗性K562细胞中积累很少,仅显示轻微的绿色荧光[4]。

[参考文献]

[1]. A Perelman, et al. JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry. Cell Death Dis. 2012 Nov 22;3:e430.

[2]. Vera C. Keil, et al. Ratiometric high-resolution imaging of JC-1 fluorescence reveals the subcellular heterogeneity of astrocytic mitochondria. Pflügers Archiv - European Journal of Physiology. 2011,462(5): 693-708.

[3]. Jung-Ho LEE, In-Hwan LEE, Young-Jun CHOE, et al. Real-time analysis of amyloid fibril formation of α-synuclein using a fibrillation-state-specific fluorescent probe of JC-1. Biochem. J. 2009, 418:311-323.

[4]. Salvioli S, et al. JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 1997 Jul 7;411(1):77-82.

[相关活性小分子]

CBIC2物理化学性质

[ 分子式 ]:
C₂₅H₂₇Cl₄IN₄

[ 分子量 ]:
652.225

[ 精确质量 ]:
650.003418

[ PSA ]:
18.67000

[ LogP ]:
4.15720

[ 储存条件 ]:
-20℃

CBIC2MSDS

详细MSDS(安全技术说明书)

CBIC2安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危险品运输编码 ]:
NONH for all modes of transport

CBIC2合成路线

详细合成路线

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CBIC2文献

Omega-3 fatty acids, EPA and DHA induce apoptosis and enhance drug sensitivity in multiple myeloma cells but not in normal peripheral mononuclear cells.

J. Nutr. Biochem. 25(12) , 1254-62, (2014)

The n-3 polyunsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been shown to enhance the effect of chemotherapeutic drugs in clinical studies in cancer patients an...

Mitochondrial targeting of bilirubin regulatory enzymes: An adaptive response to oxidative stress.

Toxicol. Appl. Pharmacol. 282(1) , 77-89, (2015)

The intracellular level of bilirubin (BR), an endogenous antioxidant that is cytotoxic at high concentrations, is tightly controlled within the optimal therapeutic range. We have recently described a ...

DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity.

J. Exp. Med. 211(13) , 2549-66, (2014)

DOCK8 mutations result in an inherited combined immunodeficiency characterized by increased susceptibility to skin and other infections. We show that when DOCK8-deficient T and NK cells migrate throug...

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