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EMA[单叠氮化溴乙非啶]

EMA[单叠氮化溴乙非啶]用途

单叠氮溴化乙锭是一种插入DNA的荧光染料,通过受损的膜进入细菌。单叠氮溴化乙锭可以通过光活化与DNA共价连接。单叠氮溴化乙锭只染色死细胞[1]。

EMA[单叠氮化溴乙非啶]名称

[ CAS 号 ]:
58880-05-0

[ 中文名 ]:
溴化乙锭单叠氮溴

[ 英文名 ]:
EMA [Ethidium monoazide bromide]

[英文别名 ]:

MFCD00213986
Ethidium azide bromide
Ethidium bromide monoazide
Ethidium monoazide bromide
Monoazido ethidium bromide

EMA[单叠氮化溴乙非啶]生物活性

[ 描述 ]:

单叠氮溴化乙锭是一种插入DNA的荧光染料,通过受损的膜进入细菌。单叠氮溴化乙锭可以通过光活化与DNA共价连接。单叠氮溴化乙锭只染色死细胞[1]。

[ 相关类别 ]:

研究领域 >> 其他

信号通路 >> 其他 >> 其他

[参考文献]

[1]. Nogva HK, et al. Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR. Biotechniques. 2003 Apr;34(4):804-8, 810, 812-3.

EMA[单叠氮化溴乙非啶]物理化学性质

[ 分子式 ]:
C₂₁H₁₈BrN₅

[ 分子量 ]:
420.30500

[ 精确质量 ]:
419.07500

[ PSA ]:
79.65000

[ LogP ]:
2.52936

[ 外观性状 ]:
固体

[ 储存条件 ]:
20°C，避光，干燥

EMA[单叠氮化溴乙非啶]MSDS

详细MSDS(安全技术说明书)

EMA[单叠氮化溴乙非啶]毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SF7941850

CHEMICAL NAME :: Phenanthridinium, 3-amino-8-azido-5-ethyl-6-phenyl-, bromide

CAS REGISTRY NUMBER :: 58880-05-0

LAST UPDATED :: 199707

DATA ITEMS CITED :: 2

MOLECULAR FORMULA :: C21-H18-N5.Br

MOLECULAR WEIGHT :: 420.35

WISWESSER LINE NOTATION :: T B666 HKJ EZ H2 IR& LNNN &E

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

MUTATION DATA

TYPE OF TEST :: Mutation in microorganisms

TEST SYSTEM :: Yeast - Saccharomyces cerevisiae

DOSE/DURATION :: 2 umol/L/30M

REFERENCE :: MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 56,21,1977

EMA[单叠氮化溴乙非啶]安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危险品运输编码 ]:
NONH for all modes of transport

[ RTECS号 ]:
SF7941850

EMA[单叠氮化溴乙非啶]文献

Modified vaccinia virus Ankara-infected dendritic cells present CD4+ T-cell epitopes by endogenous major histocompatibility complex class II presentation pathways.

J. Virol. 89(5) , 2698-709, (2015)

CD4(+) T lymphocytes play a central role in the immune system and mediate their function after recognition of their respective antigens presented on major histocompatibility complex II (MHCII) molecul...

Propidium monoazide (PMA) and ethidium bromide monoazide (EMA) improve DNA array and high-throughput sequencing of porcine reproductive and respiratory syndrome virus identification.

J. Virol. Methods 222 , 182-91, (2015)

Pan-viral DNA array (PVDA) and high-throughput sequencing (HTS) are useful tools to identify novel viruses of emerging diseases. However, both techniques have difficulties to identify viruses in clini...

Extracellular matrices decellularized from embryonic stem cells maintained their structure and signaling specificity.

Tissue Eng. Part A 20(1-2) , 54-66, (2014)

Embryonic stem cells (ESCs) emerge as a promising tool for tissue engineering and regenerative medicines due to their extensive self-renewal ability and the capacity to give rise to cells from all thr...

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