T4 DNA连接酶
T4 DNA连接酶结构式
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常用名 | T4 DNA连接酶 | 英文名 | DNA ligase |
|---|---|---|---|---|
| CAS号 | 9015-85-4 | 分子量 | 233.268 | |
| 密度 | 1.2±0.1 g/cm3 | 沸点 | 402.3±35.0 °C at 760 mmHg | |
| 分子式 | C15H11N3 | 熔点 | N/A | |
| MSDS | 中文版 美版 | 闪点 | 182.5±18.9 °C |
T4 DNA连接酶用途T4 DNA连接酶可在双链体DNA的相邻多核苷酸的末端之间形成能量依赖性的磷酸二酯键。连接反应需要ATP作为辅助因子。平末端片段的连接需要更高的酶浓度并可通过在反应混合物中使用PEG来促进。该酶需要3′羟基和5′磷酸基用于连接。可通过用碱性磷酸酶进行去磷酸化来防止载体DNA的自连。T4连接酶在DNA和RNA缺口的修复中可发挥积极作用。 |
| 中文名 | T4 DNA连接酶 |
|---|---|
| 英文名 | 2,2′:6′,2″-Terpyridine |
| 英文别名 | 更多 |
| 密度 | 1.2±0.1 g/cm3 |
|---|---|
| 沸点 | 402.3±35.0 °C at 760 mmHg |
| 分子式 | C15H11N3 |
| 分子量 | 233.268 |
| 闪点 | 182.5±18.9 °C |
| 精确质量 | 233.095291 |
| LogP | 2.13 |
| 外观性状 | 液体 |
| 蒸汽压 | 0.0±0.9 mmHg at 25°C |
| 折射率 | 1.615 |
| 储存条件 | 20°C |
| 个人防护装备 | Eyeshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US) |
|---|---|
| 危险品运输编码 | NONH for all modes of transport |
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An inhibitor of nonhomologous end-joining abrogates double-strand break repair and impedes cancer progression.
Cell 151(7) , 1474-87, (2012) DNA Ligase IV is responsible for sealing of double-strand breaks (DSBs) during nonhomologous end-joining (NHEJ). Inhibiting Ligase IV could result in amassing of DSBs, thereby serving as a strategy to... |
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One-step highly sensitive florescence detection of T4 polynucleotide kinase activity and biological small molecules by ligation-nicking coupled reaction-mediated signal amplification.
Biosens. Bioelectron. 47 , 218-24, (2013) DNA phosphorylation, catalyzed by polynucleotide kinase (PNK), plays significant regulatory roles in many biological events. Herein, using T4 PNK as a model target, we describe a one-step, highly sens... |
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Suppression of Ku70/80 or Lig4 leads to decreased stable transformation and enhanced homologous recombination in rice.
New Phytol. 196(4) , 1048-59, (2012) Evidence for the involvement of the nonhomologous end joining (NHEJ) pathway in Agrobacterium-mediated transferred DNA (T-DNA) integration into the genome of the model plant Arabidopsis remains inconc... |
| tcmdc-123985 |
| terpy |
| 2,2',2''-Terpyridine |
| 2,2':6',2''-Terpyridine |
| Terpyridine |
