蔗糖磷酸化酶

蔗糖磷酸化酶用途

蔗糖磷酸化酶属于糖苷水解酶，GH13 家族。它包含 4 个结构域，分别与葡萄糖端基碳结合站点和葡萄糖苷结合站点结合。活性站点残基包括 Asp192 和 Glu232。它主要由双歧杆菌和乳酸菌产生。交联的蔗糖磷酸化酶聚集体是耐热的，可用于糖基化的工业催化。

蔗糖磷酸化酶名称

[ CAS 号 ]:
9074-06-0

[ 中文名 ]:
蔗糖磷酸化酶

[ 英文名 ]:
SUCROSE PHOSPHORYLASE

[英文别名 ]:

MFCD00132397

蔗糖磷酸化酶生物活性

[ 描述 ]:

蔗糖磷酸化酶是一种细菌转葡糖苷酶,催化蔗糖和磷酸盐转化为α-D-葡萄糖-1-磷酸和D-果糖。葡糖基化的蔗糖磷酸化酶也可以水解成α-D-葡萄糖,或将葡糖基转移到受体的羟基,然后分解成新的α-D-葡萄糖苷产物。碱性磷酸化酶对底物和产物的酶活性较弱[1]。

[ 相关类别 ]:

研究领域 >> 其他

[参考文献]

[1]. Goedl C, et al. Sucrose phosphorylase: a powerful transglucosylation catalyst for synthesis of α-D-glucosides as industrial fine chemicals[J]. Biocatalysis and biotransformation, 2010, 28(1): 10-21.

蔗糖磷酸化酶物理化学性质

[ 密度 ]:
1.4±0.1 g/cm3

[ 沸点 ]:
584.0±60.0 °C at 760 mmHg

[ 分子式 ]:
C₂₁H₁₅F₃N₂O₂S

[ 分子量 ]:
416.416

[ 闪点 ]:
307.0±32.9 °C

[ 精确质量 ]:
416.080627

[ LogP ]:
5.56

[ 外观性状 ]:
冻干粉

[ 蒸汽压 ]:
0.0±1.6 mmHg at 25°C

[ 折射率 ]:
1.610

[ 储存条件 ]:
20°C

蔗糖磷酸化酶安全信息

[ 危险品运输编码 ]:
NONH for all modes of transport

蔗糖磷酸化酶文献

Positively charged mini-protein Zbasic2 as a highly efficient silica binding module: opportunities for enzyme immobilization on unmodified silica supports.

Langmuir 28(26) , 10040-9, (2012)

Silica is a highly attractive support material for protein immobilization in a wide range of biotechnological and biomedical-analytical applications. Without suitable derivatization, however, the sili...

Phosphorolytic cleavage of sucrose by sucrose-grown ruminal bacterium Pseudobutyrivibrio ruminis strain k3.

Folia Microbiol. (Praha) 55(4) , 383-5, (2010)

Rumen bacterium Pseudobutyrivibrio ruminis strain k3 utilized over 90% sucrose added to the growth medium as a sole carbon source. Zymographic studies of the bacterial cell extract revealed the presen...

Enzymatic synthesis of stable, odorless, and powdered furanone glucosides by sucrose phosphorylase.

Biosci. Biotechnol. Biochem. 64 , 134, (2000)

Sucrose phosphorylase from Leuconostoc mesenteroides catalyzed transglucosylation from sucrose to 4-hydroxy-3(2H)-furanone derivatives. When 4-hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF) and 2-ethyl-4-...

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