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外切核酸酶III

更新时间:2024-01-13 20:07:02

外切核酸酶III结构式
外切核酸酶III结构式
委托求购
常用名 外切核酸酶III 英文名 ExonucleaseIII
CAS号 9037-44-9 分子量 N/A
密度 N/A 沸点 N/A
分子式 N/A 熔点 N/A
MSDS 中文版 美版 闪点 N/A

 外切核酸酶III名称

中文名 外切核酸酶III
英文名 ExonucleaseIII
英文别名 更多

 外切核酸酶III物理化学性质

储存条件

-20°C密闭,避光,通风干燥处

稳定性

Biochem/physiol Actions A 3′→5′ exonuclease which catalyzes the removal of mononucleotides from the 3′-end of dsDNA. The enzyme also has apurinic and apyrimidinic endonuclease, 3′-DNA phosphatase, and RNase H activities. Activity is strongly dependent on temperature, salt concentration, and the ratio of enzyme to DNA; reaction conditions must be optimized for specific applications.

Features and Benefits • Production of strand specific probes1

• Preparation of single stranded templates for dideoxy sequencing2

• Site directed mutagenesis3

Unit Definition One unit releases 1 nmole of acid-soluble nucleotides from sonicated calf thymus DNA in 30 min at 37 °C .

Physical form Solution in 5 mM potassium phosphate pH 6.5, 200 mM KCl, 0.05 mM EDTA, 5 mM 2-mercaptoethanol, 200 μg/ml BSA, and 50% glycerol

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1. 性状:丙三醇缓冲溶液。

2. 密度(g/mL, 25 ℃ ):未确定

3. 相对蒸汽密度(g/mL,空气=1):未确定

4. 熔点(ºC):未确定

5. 沸点(ºC,常压):未确定

6. 沸点(ºC,5.2kPa):未确定

7. 折射率:未确定

8. 闪点(ºC):未确定

9. 比旋光度(º):未确定

10. 自燃点或引燃温度(ºC):未确定

11. 蒸气压(kPa,20ºC):未确定

12. 饱和蒸气压(kPa,60ºC):未确定

13. 燃烧热(KJ/mol):未确定

14. 临界温度(ºC):未确定

15. 临界压力(KPa):未确定

16. 油水(辛醇/水)分配系数的对数值:未确定

17. 爆炸上限(%,V/V):未确定

18. 爆炸下限(%,V/V):未确定

19. 溶解性:溶于水。

 外切核酸酶III安全信息

个人防护装备 Eyeshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US)
危险品运输编码 NONH for all modes of transport

 外切核酸酶III文献40

更多文献
Combined micrococcal nuclease and exonuclease III digestion reveals precise positions of the nucleosome core/linker junctions: implications for high-resolution nucleosome mapping.

J. Mol. Biol. 425(11) , 1946-60, (2013)

Micrococcal nuclease (MNase) is extensively used in genome-wide mapping of nucleosomes but its preference for AT-rich DNA leads to errors in establishing precise positions of nucleosomes. Here, we sho...

Graphene oxide-based biosensor for sensitive fluorescence detection of DNA based on exonuclease III-aided signal amplification.

Anal. Chim. Acta 727 , 67-70, (2012)

Based on the super fluorescence quenching efficiency of graphene oxide and exonuclease III aided signal amplification, we develop a facile, sensitive, rapid and cost-effective method for DNA detection...

Two-step, PCR-free telomerase detection by using exonuclease III-aided target recycling.

ChemBioChem. 12(18) , 2745-7, (2011)

We report the sensitive detection of telomerase activity by using exonuclease III-aided target recycling to amplify the signal produced by a chimeric LNA-DNA molecular beacon. We demonstrate the speci...

 外切核酸酶III英文别名

MFCD00163580