外切核酸酶III结构式
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常用名 | 外切核酸酶III | 英文名 | ExonucleaseIII |
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CAS号 | 9037-44-9 | 分子量 | N/A | |
密度 | N/A | 沸点 | N/A | |
分子式 | N/A | 熔点 | N/A | |
MSDS | 中文版 美版 | 闪点 | N/A |
中文名 | 外切核酸酶III |
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英文名 | ExonucleaseIII |
英文别名 | 更多 |
储存条件 | -20°C密闭,避光,通风干燥处 |
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稳定性 | Biochem/physiol Actions A 3′→5′ exonuclease which catalyzes the removal of mononucleotides from the 3′-end of dsDNA. The enzyme also has apurinic and apyrimidinic endonuclease, 3′-DNA phosphatase, and RNase H activities. Activity is strongly dependent on temperature, salt concentration, and the ratio of enzyme to DNA; reaction conditions must be optimized for specific applications. Features and Benefits • Production of strand specific probes1 • Preparation of single stranded templates for dideoxy sequencing2 • Site directed mutagenesis3 Unit Definition One unit releases 1 nmole of acid-soluble nucleotides from sonicated calf thymus DNA in 30 min at 37 °C . Physical form Solution in 5 mM potassium phosphate pH 6.5, 200 mM KCl, 0.05 mM EDTA, 5 mM 2-mercaptoethanol, 200 μg/ml BSA, and 50% glycerol |
更多 | 1. 性状:丙三醇缓冲溶液。 2. 密度(g/mL, 25 ℃ ):未确定 3. 相对蒸汽密度(g/mL,空气=1):未确定 4. 熔点(ºC):未确定 5. 沸点(ºC,常压):未确定 6. 沸点(ºC,5.2kPa):未确定 7. 折射率:未确定 8. 闪点(ºC):未确定 9. 比旋光度(º):未确定 10. 自燃点或引燃温度(ºC):未确定 11. 蒸气压(kPa,20ºC):未确定 12. 饱和蒸气压(kPa,60ºC):未确定 13. 燃烧热(KJ/mol):未确定 14. 临界温度(ºC):未确定 15. 临界压力(KPa):未确定 16. 油水(辛醇/水)分配系数的对数值:未确定 17. 爆炸上限(%,V/V):未确定 18. 爆炸下限(%,V/V):未确定 19. 溶解性:溶于水。 |
个人防护装备 | Eyeshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US) |
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危险品运输编码 | NONH for all modes of transport |
Combined micrococcal nuclease and exonuclease III digestion reveals precise positions of the nucleosome core/linker junctions: implications for high-resolution nucleosome mapping.
J. Mol. Biol. 425(11) , 1946-60, (2013) Micrococcal nuclease (MNase) is extensively used in genome-wide mapping of nucleosomes but its preference for AT-rich DNA leads to errors in establishing precise positions of nucleosomes. Here, we sho... |
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Graphene oxide-based biosensor for sensitive fluorescence detection of DNA based on exonuclease III-aided signal amplification.
Anal. Chim. Acta 727 , 67-70, (2012) Based on the super fluorescence quenching efficiency of graphene oxide and exonuclease III aided signal amplification, we develop a facile, sensitive, rapid and cost-effective method for DNA detection... |
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Two-step, PCR-free telomerase detection by using exonuclease III-aided target recycling.
ChemBioChem. 12(18) , 2745-7, (2011) We report the sensitive detection of telomerase activity by using exonuclease III-aided target recycling to amplify the signal produced by a chimeric LNA-DNA molecular beacon. We demonstrate the speci... |
MFCD00163580 |