TMR Biocytin

Modify Date: 2024-04-02 18:57:05

TMR Biocytin Structure
TMR Biocytin structure
Common Name TMR Biocytin
CAS Number 749247-49-2 Molecular Weight 869.08
Density N/A Boiling Point N/A
Molecular Formula C46H60N8O7S Melting Point N/A
MSDS N/A Flash Point N/A

 Use of TMR Biocytin


TMR Biocytin is a polar tracer used in the research of cell-cell and cell-liposome fusions, as well as membrane permeability and cellular uptake during pinocytosis. TMR Biocytin can be detected using streptavidin, and is an effective neuronal tracer in live tissue (Ex=544 nm, Em=571 nm)[1].

 Names

Name TMR Biocytin

 TMR Biocytin Biological Activity

Description TMR Biocytin is a polar tracer used in the research of cell-cell and cell-liposome fusions, as well as membrane permeability and cellular uptake during pinocytosis. TMR Biocytin can be detected using streptavidin, and is an effective neuronal tracer in live tissue (Ex=544 nm, Em=571 nm)[1].
Related Catalog
In Vivo TMR Biocytin can be used to examine the permeability changes of blood brain barrier (BBB)[2]. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2]. 1. Dilute 1 mg TMR Biocytin in 100 μL PBS (per mouse), and inject the solution into the tail vein. 2. 30 min after injection, anesthetize and perfuse the animals. 3. Remove spinal cords, prepare deep frozen and serial 10 μm longitudinal sections. 4. Stain nuclear counterstain using DAPI. 5. Obtaine the images of whole sections with ×10 power of objective, ×10 power of eyepiece, by using identical laser intensity, exposure times and magnification in all cohorts. 6. To set the above parameters, livers from tracer injected mice and non-injected mice were used.
References

[1]. Mads Harsløf, et al. Fast neuronal labeling in live tissue using a biocytin conjugated fluorescent probe. J Neurosci Methods. 2015 Sep 30;253:101-9.

[2]. Smadar Goldfarb, et al. Electric neurostimulation regulates microglial activation via retinoic acid receptor α signaling. Brain Behav Immun. 2021 Aug;96:40-53.

 Chemical & Physical Properties

Molecular Formula C46H60N8O7S
Molecular Weight 869.08