Carbonic anhydrase activators. The first activation study of a coral secretory isoform with amino acids and amines.

Anthony Bertucci, Didier Zoccola, Sylvie Tambutté, Daniela Vullo, Claudiu T Supuran

Index: Bioorg. Med. Chem. 18 , 2300-3, (2010)

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Abstract

The activity of the coral Stylophora pystillata secretory carbonic anhydrase STPCA has been tested in presence of amino acids and amines. All the investigated compounds showed a positive, activating effect on k(cat) and have been separated in weak (K(A) in the range of 21-126 microM), medium (10.1-19 microM) and strong enzyme activators (K(A) of 0.18-3.21 microM). D-DOPA was found to be the best coral enzyme activator, with an activation constant K(A) of 0.18 microM. This enhancement of STPCA activity, as well as previous enzyme inhibition results, might now be tested on living organisms to better understand the role played by these enzymes in the coral calcification processes.Copyright 2010 Elsevier Ltd. All rights reserved.