A refined characterisation of the NeoHepatocyte phenotype necessitates a reappraisal of the transdifferentiation hypothesis

Paloma Riquelme, Judith Wundt, James A. Hutchinson, Marc Brulport, Yu Jun, Anna Sotnikova, Ulrich Girreser, Felix Braun, Felix Gövert, Bernat Soria, Andreas Nüssler, Bernd Clement, Jan G. Hengstler, Fred Fändrich

Index: Differentiation 77(3) , 263-76, (2009)

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Abstract

Under certain culture conditions human peripheral blood monocytes may be induced to express phenotypic markers of non-haematopoietic lineages, including hepatocyte-defining traits. One such example, the NeoHepatocyte, was previously shown to express a broad panel of hepatocyte-like marker antigens and metabolic activities, both in vitro and following engraftment in the liver of immunodeficient mice. In this report, a refined description of NeoHepatocytes, with regard to their expression of xenobiotic-metabolising enzymes, morphology, hepatocyte marker expression and cell surface phenotype, is presented in comparison with human macrophages in defined states of activation. Contrary to prior assertions, it would seem more likely that NeoHepatocytes express particular hepatocyte-defining genes during a normal programme of macrophage differentiation rather than undergoing a process of transdifferentiation to become hepatocyte-like cells.