The N-terminal region of two-pore channel 1 regulates trafficking and activation by NAADP.

Dev Churamani, Robert Hooper, Taufiq Rahman, Eugen Brailoiu, Sandip Patel

Index: Biochem. J. 453(1) , 147-51, (2013)

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Abstract

TPCs (two-pore channels) are NAADP (nicotinic acid-adenine dinucleotide phosphate)-sensitive Ca2+-permeable ion channels expressed on acidic organelles. In the present study we show that deletion of the N-terminal region redirects TPC1 to the ER (endoplasmic reticulum). The introduction of fluorophores at the N-terminus of TPC1 does not affect its subcellular location, but does reversibly abolish NAADP sensitivity. Our results reveal a dual role for the N-terminus in localization and function of TPC1.