Activation of platelet-activating factor receptor in SZ95 sebocytes results in inflammatory cytokine and prostaglandin E2 production.
Qiwei Zhang, Holger Seltmann, Christos C Zouboulis, Jeffrey B Travers
Index: Exp. Dermatol. 15(10) , 769-74, (2006)
Full Text: HTML
Abstract
Platelet-activating factor (PAF) is a group of phosphocholines with various biological effects mediated by the PAF receptor (PAF-R). Activation of the epidermal PAF-R induces the expression of inflammatory mediators, including cyclooxygenase-2 (COX-2) and prostaglandin E(2) (PGE(2)). The upregulation of COX-2 expression has been shown to be involved in sebocyte proliferation, sebaceous gland inflammation and carcinogenesis. The present study was designed to investigate whether PAF-R activation could induce the expression of COX-2 and production of PGE(2), as well as secretion of the inflammatory cytokine, interleukin-8 (IL-8), in the immortalized sebaceous gland cell line SZ95. Using calcium mobilization studies, we first confirmed that PAF can signal through PAF-R in SZ95 sebocytes. We then found that the production of IL-8 was induced following treatment with PAF-R agonist, however blocked by a specific PAF-R antagonist. Induction of COX-2 expression and increased PGE(2) production were observed in SZ95 sebocytes after PAF-R activation. Finally, it was demonstrated that the production of PGE(2), induced by PAF-R activation and mediated by COX-2 expression, was blocked following PAF-R antagonism in SZ95 sebocytes. These studies suggest that SZ95 sebocytes express functional PAF-Rs and PAF-Rs are involved in regulating the expression of inflammatory mediators, including COX-2, PGE(2) and IL-8.
Related Compounds
Related Articles:
2005-10-01
[J. Clin. Invest. 115(10) , 2855-61, (2005)]
Platelet-activating factor biosynthesis induced by various stimuli in human HaCaT keratinocytes.
1996-07-01
[J. Invest. Dermatol. 107(1) , 88-94, (1996)]
2006-10-20
[J. Biol. Chem. 281(42) , 31495-501, (2006)]
2000-08-01
[J. Invest. Dermatol. 115(2) , 267-72, (2000)]
Platelet-activating factor induces collagenase expression in corneal epithelial cells.
1993-09-15
[Proc. Natl. Acad. Sci. U. S. A. 90(18) , 8678-82, (1993)]