Guanosine 5'-triphosphate-5'-adenosine

Guanosine 5'-triphosphate-5'-adenosine, the 5′ cap analog, is a fluorescent substrate analog[1].

Research Areas >> Others

Signaling Pathways >> Others >> Others

Guanosine 5'-triphosphate-5'-adenosine (GpppA) labeled with pyrene at the 3′‐O position of adenosine acted as an artificial substrate[1]. Fluorescently labeled Guanosine 5'-triphosphate-5'-adenosine (GpppA) and GpppG analogs as potential substrates that represent a reasonable compromise between the structural complexity and requirements of the enzyme[1].

[1]. Renata Kasprzyk, et al. Direct High-Throughput Screening Assay for mRNA Cap Guanine-N7 Methyltransferase Activity. Chemistry. 2020 Sep 1;26(49):11266-11275.

[2]. Dennis Reichert, et al. Light-control of cap methylation and mRNA translation via genetic code expansion of Ecm1. Chem Sci. 2021 Feb 8;12(12):4383-4388.