NPPB

[Description]:

NPPB is a blocker of the outwardly rectifying chloride channel (ORCC).

[Related Catalog]:

Signaling Pathways >> Membrane Transporter/Ion Channel >> Chloride Channel

Research Areas >> Neurological Disease

[Target]

ORCC[1]

[In Vitro]

0.1 mM NPPB in the bath solution reduces channel open probability from 0.89±0.06 to 0.11±0.04 (n=5, P<0.01)[1]. Dose-dependent inhibition of chloride currents is observed with a 50% inhibitory concentration (IC50) of 125 μM NPPB. NPPB itself also shows cytotoxicity against glioma cells with a GI50 of approximately 500 μM[2].

[Cell Assay]

Cells are seeded in the 96-well microtiter plate at a density of 5×103 cells per well and incubated at 37°C for 24 h in a humidified 5% CO2 atmosphere. After removing the culture medium, fresh media containing various concentrations of NPPB is added, and incubated for 24 h. Next, 100 μL of Thiazolyl blue tetrazolium bromide at 0.5 mg/mL is added to each well and incubated at 37°C for 1 h. Cells are then dissolved in 100 μL of DMSO, and the absorbance is measured at 570 nm with a Microplate Reader. Concentration-response curves of NPPB are fitted to a Hill equation to obtain GI50 and GI80 (50% and 80% growth inhibition concentrations, respectively) values[2].

[References]

[1]. Li J, et al. Enhancement of an outwardly rectifying chloride channel in hippocampal pyramidal neurons after cerebral ischemia. Brain Res. 2016 Aug 1;1644:107-17.

[2]. Park M, et al. Double Blockade of Glioma Cell Proliferation and Migration by Temozolomide Conjugated withNPPB, a Chloride Channel Blocker. ACS Chem Neurosci. 2016 Mar 16;7(3):275-85.

[Related Small Molecules]

[ Density]:
1.323 g/cm3

[ Boiling Point ]:
523.5ºC at 760 mmHg

[ Melting Point ]:
178-179ºC

[ Molecular Formula ]:
C₁₆H₁₆N₂O₄

[ Molecular Weight ]:
300.30900

[ Exact Mass ]:
300.11100

[ PSA ]:
95.15000

[ LogP ]:
3.93390

[ Vapour Pressure ]:
8.73E-12mmHg at 25°C

[ Index of Refraction ]:
1.653

[ Storage condition ]:
2-8℃

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[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

[ HS Code ]:
2922499990

[ HS Code ]: 2922499990

[ Summary ]:
HS:2922499990 other amino-acids, other than those containing more than one kind of oxygen function, and their esters; salts thereof VAT:17.0% Tax rebate rate:9.0% Supervision conditions:AB(certificate of inspection for goods inward,certificate of inspection for goods outward) MFN tariff:6.5% General tariff:30.0%

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