Z-LEVD-FMK

Z-LEVD-FMK is a cell-permeable caspase-4 inhibitor. Z-LEVD-FMK blocks ER stress-induced apoptosis in cancer cells[1].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Signaling Pathways >> Apoptosis >> Caspase

Caspase-4

Z-LEVD-FMK (2 ng/mL, 30 min) blocks IL-1β-induced IL-8 production in hRPE cells[1]. Z-LEVD-FMK (2 μM) inhibits caspase-3 activity in hRPE cells[1]. Z-LEVD-FMK (20 μM, 96 h) completely blocks E2-induced PARP cleavage in 5C cells[2]. Z-LEVD-FMK (20 μM, 96 h) reverses E2-inhibited growth and prevents morphologic alterations associated with apoptosis in 5C cells[2]. Western Blot Analysis[1] Cell Line: hRPE cells (treated with IL-1β of 2 ng/mL for additional 24 h) Concentration: 2 ng/mL Incubation Time: 30 min Result: Inhibited IL-1β-induced IL-8 production.

[1]. Bian ZM, et al. Dual involvement of caspase-4 in inflammatory and ER stress-induced apoptotic responses in human retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 2009 Dec;50(12):6006-14.  

[2]. Ariazi EA, et al. Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time. Proc Natl Acad Sci U S A. 2011 Nov 22;108(47):18879-86.