N-BIOTINYL-N'-MALEIMIDO-ETHYLENEDIAMINE

Biotin-C2-maleimide is an antibody conjugate reagent, can bind to many biomolecules without significantly changing the biological activity of the target molecule[1][2].

Research Areas >> Cancer

Signaling Pathways >> Others >> Others

Biotin-C2-maleimide () can act bioconjugation with 5 eq. SUMO-1 probes[1]. 1. Biotin C2 maleimide (ATT Bioquest, dissolved in DMSO) is performed in Tris buffer (50 mM Tris, 300 mM NaCl; pH 7.0) after 15 min reduction with Tris (2-carboxyethyl)phosphine (TCEP, 120 µM) at room temperature. 2. After 1 h incubation, the unreacted Biotin is separated by Ni-NTA affinity chromatography followed by dialysis against PBS buffer (pH 7.4).

[1]. Benjamin GD, et al. Antibody conjugates and methods of making the antibody conjugates: United States, US20160361436A1. 2016-12-15.

[2]. Brüninghoff K, Wulff S, Dörner W, Geiss-Friedlander R, Mootz HD. A Photo-Crosslinking Approach to Identify Class II SUMO-1 Binders. Front Chem. 2022 May 30;10:900989.