Mastoparan 7 trifluoroacetate salt

[Description]:

Mas7, a structural analogue of mastoparan, is an activator of heterotrimeric Gi proteins and its downstream effectors.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Research Areas >> Cardiovascular Disease

Research Areas >> Neurological Disease

Peptides

[In Vitro]

Mas7 produces several biological effects in different cell types. The effect of Mas7 on endogenous mono-ADP-ribosyltransferase activity is in the micromolar range with a maximal activation of 205% over the basal. In pertussis treated plasma membranes, it is found that the effect of Mas7 on endogenous mono-ADP-ribosyltransferase is partially blocked, which suggests the involvement of G-proteins, such as Gi or G0[1]. Mas7 is a basic tetradecapeptide isolated from isp venom, which activates guanine nucleotide-binding regulatory proteins (G-proteins) and stimulates apoptosis. In smooth muscle cells, Mas7 leads to an increase in the perfusion pressure. Vascular contraction is induced by Mas7. The vasoconstriction triggered by mas-7 exhibited a slower increase compared to that simulated by phenylephrine or vasopressin[2]. Exposure of hippocampal neurons to a low dose of Mas-7 increases dendritic spine density and spine head width in a time-dependent manner. Additionally, Mas-7 enhances postsynaptic density protein-95 (PSD-95) clustering in neurites and activates Gαo signaling, increasing the intracellular Ca2+ concentration[3].

[Cell Assay]

Hippocampal neurons cultured in round 35 mm coverslips at a density of 160,000 cells/coverslip are transfected with EGFP at 11 DIV. Then, at 14 DIV the neurons are placed in the imaging chamber in an isotonic solution. The EGFP-positive neurons are imaged with microscope every 5 min for 45 min after the treatment with 1 μM Mas-7. The images are processed and analyzed using ImageJ software[1].

[References]

[1]. Bavec A, et al. Novel features of amphiphilic peptide Mas7 in signalling via heterotrimeric G-proteins. J Pept Sci. 2004 Nov;10(11):691-9.

[2]. Grze?k G, et al. Direct regulation of vascular smooth muscle contraction by mastoparan-7. Biomed Rep. 2014 Jan;2(1):34-38.

[3]. Ramírez VT, et al. The Gαo Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons. Neural Plast. 2016;2016:4258171.

[Related Small Molecules]

[ Density]:
1.154g/cm3

[ Boiling Point ]:
1649.9ºC at 760mmHg

[ Molecular Formula ]:
C₆₇H₁₂₄N₁₈O₁₅

[ Molecular Weight ]:
1421.81

[ Flash Point ]:
951.6ºC

[ PSA ]:
644.54000

[ LogP ]:
5.49270

[ Vapour Pressure ]:
0mmHg at 25°C

[ Index of Refraction ]:
1.523

Click to get detail MSDS

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

Activation of GTP formation and high-affinity GTP hydrolysis by mastoparan in various cell membranes. G-protein activation via nucleoside diphosphate kinase, a possible general mechanism of mastoparan action.

Biochem. Pharmacol. 51 , 217-223, (1996)

The wasp venom, mastoparan (MP), is a direct activator of reconstituted pertussis toxin-sensitive G-proteins and of purified nucleoside diphosphate kinase (NDPK) [E.C. 2.6.4.6.]. In HL-60 membranes, M...

The heterotrimeric G-protein Gi is localized to the insulin secretory granules of beta-cells and is involved in insulin exocytosis.

J. Biol. Chem. 270 , 12869, (1995)

Mastoparan, a tetradecapeptide found in wasp venom that stimulates G-proteins, increases insulin secretion from beta-cells. In this study, we have examined the role of heterotrimeric G-proteins in mas...

Mastoparan may activate GTP hydrolysis by Gi-proteins in HL-60 membranes indirectly through interaction with nucleoside diphosphate kinase.

Biochemistry 304 , 377-383, (1994)

The wasp venom, mastoparan (MP), activates reconstituted pertussis toxin (PTX)-sensitive G-proteins in a receptor-independent manner. We studied the effects of MP and its analogue, mastoparan 7 (MP 7)...

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