5-Carboxyfluorescein diacetate N-succinimidyl ester

Names

[ Name ]:
5-Carboxyfluorescein diacetate N-succinimidyl ester

[Synonym ]:
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester
Carboxyfluorescein diacetate succinimidyl ester
2,5-Pyrrolidinedione, 1-[[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]oxy]-
5(6)-CFDA (SE [5(6)-Carboxyfluorescein diacetate
MFCD00037466
5-Carboxyfluorescein diacetate N-Hydroxysuccinimide Ester
5-{[(2,5-Dioxo-1-pyrrolidinyl)oxy]carbonyl}-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetate
5-CFDA N-succinimidyl ester
5-Carboxyfluorescein diacetate N-succinimidyl ester
CFSE

Biological Activity

[Description]:

CFSE is a fluorescent dye which can track the cell division.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

[In Vitro]

CFSE is a fluorescent dye which can track the cell division[1]. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeling immune cells[2].

[Cell Assay]

Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert[2].

[References]

[1]. Cinthia C. Stempin, et al. GRAIL and Otubain-1 are Related to T Cell Hyporesponsiveness during Trypanosoma cruzi Infection. PLoS Negl Trop Dis. 2017 Jan; 11(1): e0005307.

[2]. Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6(7): e1333210.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.6±0.1 g/cm3

[ Boiling Point ]:
757.9±70.0 °C at 760 mmHg

[ Melting Point ]:
152-154ºC(lit.)

[ Molecular Formula ]:
C₂₉H₁₉NO₁₁

[ Molecular Weight ]:
557.461

[ Flash Point ]:
412.2±35.7 °C

[ Exact Mass ]:
557.095825

[ PSA ]:
151.81000

[ LogP ]:
0.50

[ Vapour Pressure ]:
0.0±2.6 mmHg at 25°C

[ Index of Refraction ]:
1.701

[ Storage condition ]:
-20°C

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi

[ RIDADR ]:
NONH for all modes of transport

Articles

MHC class II presentation is controlled by the lysosomal small GTPase, Arl8b.

J. Immunol. 194(5) , 2079-88, (2015)

Dendritic cells (DCs) are specialized APCs with the ability to prime naive T cells. DCs first sample Ags from the environment and then orchestrate their processing and loading onto MHC class II (MHC I...

Coculture of peripheral blood-derived mesenchymal stem cells and endothelial progenitor cells on strontium-doped calcium polyphosphate scaffolds to generate vascularized engineered bone.

Tissue Eng. Part A 21(5-6) , 948-59, (2015)

Vascularization of engineered bone tissue is critical for ensuring its survival after implantation and it is the primary factor limiting its clinical use. A promising approach is to prevascularize bon...

Olanzapine inhibits proliferation, migration and anchorage-independent growth in human glioblastoma cell lines and enhances temozolomide's antiproliferative effect.

J. Neurooncol. 122(1) , 21-33, (2015)

The poor prognosis of patients with glioblastoma fuels the search for more effective therapeutic compounds. We previously hypothesised that the neuroleptic olanzapine may enhance antineoplastic effect...