Di-8-ANEPPS

Names

[ Name ]:
Di-8-ANEPPS

[Synonym ]:
3-[4-[(E)-2-[6-(dioctylamino)naphthalen-2-yl]ethenyl]pyridin-1-ium-1-yl]propane-1-sulfonate
Di-8-ANEPPS

Biological Activity

[Description]:

Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm .

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

[In Vitro]

A mouse cell staining with Di-8-ANEPPS is applied voltage clamp pulses and immersed in Na+-containing solution. The Di-8-ANEPPS signal, which largely reflects t system voltage, has an asymmetrical positive component upon application of depolarizing pulses. This is interpreted as reflecting a propagating action potential, escaping from the voltage-clamped plasma membrane. Its temporal properties are not unlike those of the “center” signal. Notably, the peak of the signal occurrs 0.3 ms after the leading edge of the depolarizing pulse[1].

[Cell Assay]

To implement the technique, cells enzymatically isolated from mouse flexor digitorum brevis (FDB) muscle are stained by brief exposure to a saline with Di-8-ANEPPS and then washed in solution[1].

[References]

[1]. Manno C, et al. Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS. J Gen Physiol. 2013 Mar;141(3):371-87.

[Related Small Molecules]

Chemical & Physical Properties

[ Molecular Formula ]:
C₃₆H₅₂N₂O₃S

[ Molecular Weight ]:
592.87500

[ Exact Mass ]:
592.37000

[ PSA ]:
72.70000

[ LogP ]:
9.84120

[ Storage condition ]:
2-8℃

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

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Purinergic signaling may be involved in embryonic development of the heart. In the present study, the effects of purinergic receptor stimulation on cardiomyogenesis of mouse embryonic stem (ES) cells ...

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Differentiation of pluripotent human embryonic stem cells (hESCs) to the cardiac lineage represents a potentially unlimited source of ventricular cardiomyocytes (VCMs), but hESC-VCMs are developmental...

Optical signals from neurons with internally applied voltage-sensitive dyes.

J. Neurosci. 15 , 1392, (1995)

We carried out experiments to monitor optically the generation and spread of action potentials and subthreshold potentials in the processes of individual neurons in ganglia of the snail, Helix aspersa...