NAI-N3
Names
[ CAS No. ]:
1612756-29-2
[ Name ]:
NAI-N3
Biological Activity
[Description]:
NAI-N3 is a novel RNA acylation reagent that enables RNA purification.
[Related Catalog]:
[In Vitro]
The bifunctional NAI-N3 SHAPE reagent is used to label all RNAs inside living cells (or in vitro refolded RNA) to generate covalent modifications on targeted RNAs at single-nucleotide resolution. RNA is isolated, biotinylated using click chemistry and randomly fragmented to a size of 50–100 nt. To generate cDNA sequencing libraries, the RNA fragments are subjected to 3′-end repair, adapter ligation, size selection, biotin enrichment, reverse transcription (RT) and PCR. The end result is selective amplification of fragments corresponding to modified bases in the NAI-N3–treated samples or background expression values from mock-treated samples. Sequencing reads generated from the NAI-N3– and mock (DMSO)-treated samples are mapped to a transcriptome annotation, followed by computational identification of RT stops corresponding to SHAPE modifications—and hence reactive nucleotides—across the entire transcriptome[1].
[References]
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Chemical & Physical Properties
[ Molecular Formula ]:
C10H8N6O
[ Molecular Weight ]:
228.21
[ Storage condition ]:
2-8℃