FM4-64

Names

[ Name ]:
FM4-64

[Synonym ]:
Pyridinium,4-[6-[4-(diethylamino)phenyl]-1,3,5-hexatrienyl]-1-[3-(triethylammonio) propyl]-,dibromide
N-(3-Triethylammoniopropyl)-4-(6-(4-(diethylamino)phenyl) hexatrienyl)pyridinium dibromide
3-[4-[(1E,3E,5E)-6-[4-(diethylamino)phenyl]hexa-1,3,5-trienyl]pyridin-1-ium-1-yl]propyl-triethylazanium dibromide
SynaptoRed™ C2

Biological Activity

[Description]:

FM4-64, a membrane-selective red fluorescent dye, belongs to the FM family of styrylpyridinium dyes. FM4-64 is widely used to study endocytosis and exocytosis, vesicle trafficking and organelle organization in living animal[1][2].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

FM4-64 readily translocates to the nuclear envelope (NE) at physiological temperature where it exhibits enhanced fluorescence when excited at 620-650 nm in contrast to 480-520 nm excitation in the endocytic pathway and in the endoplasmic reticulum (ER). In vitro data indicated that the dye reveals an enrichment of negatively charged lipids, presumably due to local phospholipid synthesis. Dual-excitation imaging of FM4-64 in relation to lamina-associated polypeptide-1-green fluorescent protein during mitosis suggested that the disassembly of NE preserves microscale lipid complexes in the ER[1]. For studies onplant and fungal cells, FM4-64 is usually preferred to FM1-43 due to its superior brightness, greater contrast, higher photostability, and its red-shifted emission facilitating concomitantuse with the green fluorescent protein (GFP).[3]

[References]

[1]. Zal T, et al. Spectral shift of fluorescent dye FM4-64 reveals distinct microenvironment of nuclear envelope in living cells. Traffic. 2006;7(12):1607-1613.

[2]. Stenovec M, et al. Distinct labelling of fusion events in rat lactotrophs by FM 1-43 and FM 4-64 is associated with conformational differences. Acta Physiol (Oxf). 2007;191(1):35-42.

[3]. Bolte S, et al, Satiat-Jeunemaitre B. FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells. J Microsc. 2004;214(Pt 2):159-173.

Chemical & Physical Properties

[ Molecular Formula ]:
C₃₀H₄₅Br₂N₃

[ Molecular Weight ]:
607.50600

[ Exact Mass ]:
605.19800

[ PSA ]:
7.12000

[ LogP ]:
0.37770

[ Storage condition ]:
-20°C

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Safety Phrases ]:
22-24/25

[ RIDADR ]:
NONH for all modes of transport

Articles

A novel fluorescence-activated cell sorter-based screen for yeast endocytosis mutants identifies a yeast homologue of mammalian eps15.

J. Cell Biol. 135 , 1485, (1996)

A complete understanding of the molecular mechanisms of endocytosis requires the discovery and characterization of the protein machinery that mediates this aspect of membrane trafficking. A novel gene...

A cell-free assay allows reconstitution of Vps33p-dependent transport to the yeast vacuole/lysosome.

J. Cell Biol. 146 , 85, (1999)

We report a cell-free system that measures transport-coupled maturation of carboxypeptidase Y (CPY). Yeast spheroplasts are lysed by extrusion through polycarbonate filters. After differential centrif...